For the quantification of resveratrol (RV) in the Wistar rat skin, an ultra-performance liquid chromatography-mass spectrometric (UPLC–MS/MS) method was developed and validated on ACQUITY UPLC BEH C18 column (1.7 μm). The mobile phase ratio of methanol (A) and 2% formic acid (B) (ratio 10: 90% v/v, 80: 20 v/v) at isocratic elution with flow rate 0.3 mL/min, and run time 3 min was used for analysis. In addition, the use of multiple reaction monitoring (MRM)/ES+ mode to detect the analytes and to track parents to daughter ion transition of 229.17 > 107.04 m/z (time scan 3 min, retention time 1.48) for RV and curcumin as an internal standard shows 369.16 > 176.93m/z (scan time is 2.80 min, retention time is 1.11), respectively. Linearity was observed in the range of 2.5 to 2,000 ng/mL (R2 = 0.987). Precision and accuracy on rat skin were within the acceptability range (RE%: ±15; RSD%: ±15). Moreover, it showed a good percentage recovery and found within acceptance limit 90–110%. Lower limit of detection and quantitation for the method observed to be 2.5 and 20 ng.mL−1, respectively. Method application indicated successful determination of dermatokinetics parameters of RV from lipospheres gel and suspension in the rats.

中文翻译：

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UPLC-MS/MS 方法验证通过脂质球凝胶制剂估算大鼠皮肤中的白藜芦醇及其在大鼠皮肤动力学研究中的应用

为了定量 Wistar 大鼠皮肤中的白藜芦醇 (RV)，开发了一种超高效液相色谱-质谱 (UPLC-MS/MS) 方法，并在 ACQUITY UPLC BEH C18 色谱柱 (1.7 μm) 上进行了验证。甲醇 (A) 和 2% 甲酸 (B) 的流动相比例（比例 10: 90% v/v, 80: 20 v/v），等度洗脱，流速 0.3 mL/min，运行时间 3 min用于分析。此外，使用多反应监测 (MRM)/ES+ 模式来检测分析物并跟踪 229.17 > 的父离子到子离子的转换。RV 和姜黄素作为内标的 107.04 m/z（时间扫描 3 分钟，保留时间 1.48）显示 369.16 > 分别为 176.93m/z（扫描时间为 2.80 分钟，保留时间为 1.11）。在 2.5 至 2,000 ng/mL (R2 = 0.987) 范围内观察到线性。大鼠皮肤的精密度和准确度在可接受范围内（RE%：±15；RSD%：±15）。此外，它显示出良好的百分比回收率，并在可接受的范围内 90-110%。观察到的方法的检测和定量下限分别为 2.5 和 20 ng.mL-1。方法应用表明成功地测定了大鼠脂质球凝胶和悬浮液中RV的皮肤动力学参数。