The ‘Honeycrisp’ apple, an economically important cultivar and breeding parent, is prone to soft scald and soggy breakdown postharvest physiological disorders. Phenotypic evaluation of soft scald is time consuming and costly, making it an excellent target for DNA-informed breeding. The objective of this study was to develop a DNA test for a soft scald and soggy breakdown quantitative trait locus (QTL) on linkage group two (LG2) that was characterized in a previous study. ‘Honeycrisp’ is homozygous for the undesirable high disorder incidence haplotype (HDI) at this QTL. In this study, sixteen single nucleotide polymorphism markers were evaluated for their associations with the HDI haplotype in a set of 132 unique cultivars and important breeding parents. A DNA test was successfully developed utilizing KASP™ (Kompetitive Allele Specific PCR) chemistry to identify the number of HDI haplotypes in individuals. This test had a 100% accuracy for detecting homozygous unfavorable HDI individuals and has an expected 88% accuracy over all three haplotype copy groups across the evaluated germplasm. This DNA test is a promising tool for minimizing the chances of selecting individuals that exhibit high incidence of soft scald postharvest disorder in ‘Honeycrisp’-related germplasm.

“Honeycrisp”苹果是一种重要的经济品种和育种亲本，在采收后很容易出现软烫和潮湿分解的生理疾病。软烫伤的表型评估既耗时又昂贵，使其成为 DNA 知情育种的绝佳目标。本研究的目的是针对先前研究中表征的连锁群二 (LG2) 上的软烫伤和潮湿分解数量性状基因座 (QTL) 开发 DNA 测试。“Honeycrisp”对于该 QTL 处的不良高疾病发生率单倍型 (HDI) 是纯合的。在这项研究中，评估了 16 个单核苷酸多态性标记与 132 个独特品种和重要育种亲本中 HDI 单倍型的关联。利用 KASP™（竞争性等位基因特异性 PCR）化学方法成功开发了 DNA 测试，以识别个体中 HDI 单倍型的数量。该测试对于检测纯合不利 HDI 个体具有 100% 的准确度，并且对所评估种质中的所有三个单倍型拷贝组的预期准确度为 88%。这种 DNA 测试是一种很有前途的工具，可以最大限度地减少选择在“Honeycrisp”相关种质中表现出高发软烫伤采后病症的个体的机会。