Neo-tetraploid Arabidopsis thaliana have elevated leaf potassium (K) driven by processes within the root. The root transcriptome of neo-tetraploids is distinct from diploids, with evidence of altered K homeostasis. Mutational analysis revealed that the canonical K-uptake transporters AKT1 and HAK5 are not required for this elevated leaf K in neo-tetraploids, while the endodermis, root hairs, and SOS signaling are. Contrasting the root transcriptomes of neo-tetraploids and diploids of mutants that block the neo-tetraploid K phenotype, allowed us to identify 91 differentially expressed genes associated with elevated leaf K in neo-tetraploids. This set of genes connects WGD to elevated leaf K, and is enriched in functions such as cell wall and Casparian strip development, and ion-transport, in the endodermis, root hairs, and procambium. This gene set provides tools to test the intriguing idea of recreating the physiological effects of WGD within a diploid genome.

中文翻译：

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支持新四倍体中倍性相关增加叶钾的转录网络

新四倍体拟南芥由根内的过程驱动的叶钾（K）升高。新四倍体的根转录组与二倍体不同，有证据表明 K 稳态发生改变。突变分析表明，新四倍体中这种升高的叶 K 不需要典型的 K 吸收转运蛋白 AKT1 和 HAK5，而内皮、根毛和 SOS 信号则需要。对比阻断新四倍体 K 表型的新四倍体和二倍体突变体的根转录组，使我们能够鉴定 91 个与新四倍体中叶 K 升高相关的差异表达基因。这组基因将 WGD 与升高的叶 K 联系起来，并且在内皮层、根毛和原形成层中富含细胞壁和凯氏带发育以及离子传输等功能。