Flax (Linum usitatissimum L.) seed oil, which accumulates in the embryo, and mucilage, which is synthesized in the seed coat, are of great economic importance for food, pharmaceutical as well as chemical industries. Theories on the link between oil and mucilage production in seeds consist in the spatio-temporal competition of both compounds for photosynthates during the very early stages of seed development. In this study, we demonstrate a positive relationship between seed oil production and seed coat mucilage extrusion in the agronomic model, flax. Three recombinant inbred lines were selected for low, medium and high mucilage and seed oil contents. Metabolite and transcript profiling (1H NMR and DNA oligo-microarrays) was performed on the seeds during seed development. These analyses showed main changes in the seed coat transcriptome during the mid-phase of seed development (25 Days Post-Anthesis), once the mucilage biosynthesis and modification processes are thought to be finished. These transcriptome changes comprised genes that are putatively involved in mucilage chemical modification and oil synthesis, as well as gibberellic acid (GA) metabolism. The results of these integrative biology approach, suggest that transcriptional regulations of seed oil and fatty acid (FA) metabolism could occur in the seed coat during the mid-stage of seed development, once the seed coat carbon supplies have been used for mucilage biosynthesis and mechanochemical properties of the mucilage secretory cells.

中文翻译：

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亚麻种子发育中期的表皮特异性转录调控影响粘液的释放和种子油含量

亚麻（Linum usitatissimumL.) 在胚中积累的种子油和在种皮中合成的粘液对食品、制药和化学工业具有重要的经济意义。关于种子中油和粘液生产之间联系的理论包括两种化合物在种子发育的早期阶段对光合产物的时空竞争。在这项研究中，我们证明了农艺模型亚麻中种子油产量和种皮粘液挤出之间存在正相关关系。选择了低、中、高粘液和种子油含量的三个重组自交系。在种子发育过程中对种子进行代谢物和转录物分析（1H NMR 和 DNA 寡微阵列）。这些分析显示在种子发育中期（开花后 25 天），一旦粘液生物合成和修饰过程被认为完成，种皮转录组的主要变化。这些转录组变化包括假定参与粘液化学修饰和油合成以及赤霉酸 (GA) 代谢的基因。这些综合生物学方法的结果表明，一旦种皮碳供应用于粘液生物合成和粘液分泌细胞的机械化学特性。这些转录组变化包括假定参与粘液化学修饰和油合成以及赤霉酸 (GA) 代谢的基因。这些综合生物学方法的结果表明，一旦种皮碳供应用于粘液生物合成和粘液分泌细胞的机械化学特性。这些转录组变化包括假定参与粘液化学修饰和油合成以及赤霉酸 (GA) 代谢的基因。这些综合生物学方法的结果表明，一旦种皮碳供应用于粘液生物合成和粘液分泌细胞的机械化学特性。