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Quantum dots-based fluorescence immunoassay for detection of tiamulin in pork
Journal of Food Safety ( IF 1.9 ) Pub Date : 2021-09-12 , DOI: 10.1111/jfs.12930
Jingming Zhou 1 , Xiaoli Zhang 1 , Wenjing Qian 1 , Qingbao Yang 1 , Yanhua Qi 1 , Yumei Chen 1 , Aiping Wang 1
Affiliation  

Tiamulin (TML) is a kind of diterpenoid veterinary antibiotic. If abused, it will cause residues in edible tissues of animals and endanger human health. Thus, a facile, sensitive, and accurate method is essential for TML detection. In this study, TML complete antigen was synthesized by oximation and active ester method, and monoclonal antibodies with high specificity and sensitivity were prepared. ZnCdSe/ZnS quantum dots (QDs) coupled with goat anti-mouse IgG were used as a fluorescence detection probe to establish indirect competitive fluorescent immunosorbent assay (icFLISA) for the determination of TML in pork. The 50% inhibitory concentration (IC50) of the icFLISA was 0.069 ng/ml, and the limits of detection (LOD) were 0.309 pg/ml. The recovery of the spiked pork samples for the icFLISA ranged from 85.6 to 96.8%, and the coefficient of variation (CV) was less than 9.4%. The results showed that the icFLISA based on ZnCdSe/ZnS QDs was more sensitive than the traditional indirect competitive enzyme-linked immunosorbent assay for the detection of TML in edible animal tissues.

中文翻译:

基于量子点的荧光免疫法检测猪肉中的泰妙菌素

泰妙菌素(TML)是一种二萜类兽用抗生素。如果滥用,会在动物的可食用组织中产生残留,危害人体健康。因此,一种简便、灵敏且准确的方法对于 TML 检测至关重要。本研究采用肟化法和活性酯法合成TML完全抗原,制备具有高特异性和敏感性的单克隆抗体。以ZnCdSe/ZnS量子点(QDs)与山羊抗小鼠IgG为荧光检测探针,建立间接竞争荧光免疫吸附法(icFLISA)测定猪肉中的TML。50% 抑制浓度 (IC 50) 的 icFLISA 为 0.069 ng/ml,检测限 (LOD) 为 0.309 pg/ml。icFLISA 加标猪肉样品的回收率为 85.6% 至 96.8%,变异系数 (CV) 小于 9.4%。结果表明,基于 ZnCdSe/ZnS QD 的 icFLISA 比传统的间接竞争性酶联免疫吸附法检测可食用动物组织中的 TML 更灵敏。
更新日期:2021-09-12
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