The extent splicing is regulated at single-cell resolution has remained controversial due to both available data and methods to interpret it. We apply the SpliZ, a new statistical approach, to detect cell-type-specific splicing in >110K cells from 12 human tissues. Using 10x data for discovery, 9.1% of genes with computable SpliZ scores are cell-type-specifically spliced, including ubiquitously expressed genes MYL6 and RPS24. These results are validated with RNA FISH, single-cell PCR, and Smart-seq2. SpliZ analysis reveals 170 genes with regulated splicing during human spermatogenesis, including examples conserved in mouse and mouse lemur. The SpliZ allows model-based identification of subpopulations indistinguishable based on gene expression, illustrated by subpopulation-specific splicing of classical monocytes involving an ultraconserved exon in SAT1. Together, this analysis of differential splicing across multiple organs establishes that splicing is regulated cell-type-specifically.

中文翻译：

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RNA 剪接程序以单细胞分辨率定义组织区室和细胞类型


由于可用的数据和解释它的方法，单细胞分辨率下剪接的调节程度仍然存在争议。我们应用 SpliZ（一种新的统计方法）来检测来自 12 种人体组织的 >110K 细胞中的细胞类型特异性剪接。使用 10 倍数据进行发现，具有可计算 SpliZ 分数的基因中有 9.1% 是细胞类型特异性剪接的，包括普遍表达的基因MYL6和RPS24 。这些结果通过 RNA FISH、单细胞 PCR 和 Smart-seq2 进行了验证。 SpliZ 分析揭示了人类精子发生过程中 170 个剪接受调控的基因，其中包括小鼠和小鼠狐猴中保守的例子。 SpliZ 允许基于模型识别基于基因表达无法区分的亚群，这通过涉及SAT1中超保守外显子的经典单核细胞的亚群特异性剪接来说明。总之，对跨多个器官的差异剪接的分析证实剪接是细胞类型特异性调节的。