The bacterial pathogens Streptococcus agalactiae (GBS) and Staphylococcus aureus (S. aureus) cause serious infections in humans and animals. The emergence of antibiotic-resistant isolates and bacterial biofilm formation entails the urge of novel treatment strategies. Recently, there is a profound scientific interest in the capabilities of non-digestible oligosaccharides as antimicrobial and anti-biofilm agents as well as adjuvants in antibiotic combination therapies. In this study, we investigated the potential of alginate oligosaccharides (AOS) and chitosan oligosaccharides (COS) as alternative for, or in combination with antibiotic treatment. AOS (2–16%) significantly decreased GBS V growth by determining the minimum inhibitory concentration. Both AOS (8 and 16%) and COS (2–16%) were able to prevent biofilm formation by S. aureus wood 46. A checkerboard biofilm formation assay demonstrated a synergistic effect of COS and clindamycin on the S. aureus biofilm formation, while AOS (2 and 4%) were found to sensitize GBS V to trimethoprim. In conclusion, AOS and COS affect the growth of GBS V and S. aureus wood 46 and can function as anti-biofilm agents. The promising effects of AOS and COS in combination with different antibiotics may offer new opportunities to combat antimicrobial resistance.

细菌病原体无乳链球菌（GBS）和金黄色葡萄球菌（金黄色葡萄球菌）引起人类和动物的严重感染。抗生素耐药菌株的出现和细菌生物膜的形成迫切需要新的治疗策略。最近，人们对不可消化寡糖作为抗菌剂和抗生物膜剂以及抗生素联合疗法佐剂的能力产生了深刻的科学兴趣。在这项研究中，我们研究了藻酸盐寡糖 (AOS) 和壳寡糖 (COS) 作为抗生素治疗替代品或与抗生素治疗联合使用的潜力。通过确定最低抑制浓度，AOS (2–16%) 显着降低 GBS V 的生长。 AOS（8% 和 16%）和 COS（2-16%）都能够通过以下方式防止生物膜形成：金黄色葡萄球菌木材 46. 棋盘生物膜形成试验证明 COS 和克林霉素对金黄色葡萄球菌生物膜形成，而 AOS（2% 和 4%）被发现使 GBS V 对甲氧苄啶敏感。总之，AOS和COS影响GBS V和GBS V的生长金黄色葡萄球菌wood 46 可以作为抗生物膜剂。 AOS 和 COS 与不同抗生素联合使用的良好效果可能为对抗抗菌素耐药性提供新的机会。