This study aimed to determine the effect of the growth stage of Procambarus clarkii on their intestinal microbiota. Intestinal samples of five different growth stages of P. clarkii (first instar, second instar, third instar, juvenile, and adult) from laboratory culture were analyzed through the Illumina MiSeq high-throughput sequencing platform to determine the intestinal microbiome of crayfish. The alpha diversity decreased along with the growth of the crayfish, with the relative abundance of the microbiota changing among stages; crayfish at closer development stages had a more comparable intestinal microbiota composition. A comparative analysis by principal component analysis and principal coordinate analysis showed that there were significant differences in the intestinal microbiota of crayfish among the different growth stages, except for the first two stages of larval crayfish, and the intestinal microbiota showed a consistent progression pattern from the larval stage to the juvenile stage. Some microbiota showed stage specificity, which might be the characteristic microbiota of different stages of growth. According to FAPROTAX functional clustering analysis, the three stages of larvae were clustered together, while the juvenile and adult stages were clustered separately according to the growth stage, indicating that, in the early stages of larval development, the function of the intestinal flora was similar; as the body grew and developed, the composition and function of the intestinal microbiota also changed.

本研究旨在确定生长阶段的影响 克氏原螯虾在他们的肠道微生物群中。五个不同生长阶段的肠道样本克拉氏假单胞菌通过Illumina MiSeq高通量测序平台分析来自实验室培养的一龄、二龄、三龄、幼鱼和成鱼，以确定小龙虾的肠道微生物组。α多样性随着小龙虾的生长而降低，微生物群的相对丰度在不同阶段发生变化；处于较近发育阶段的小龙虾具有更相似的肠道微生物群组成。主成分分析和主坐标分析对比分析表明，除小龙虾幼体前两个阶段外，不同生长阶段小龙虾肠道菌群存在显着差异，肠道菌群从幼体开始呈现一致的进展模式。幼虫期到幼虫期。一些微生物群表现出阶段特异性，这可能是不同生长阶段的特征微生物群。根据FAPROTAX功能聚类分析，三个阶段的幼虫被聚类在一起，而幼虫和成虫阶段根据生长阶段分别聚类，表明在幼虫发育的早期阶段，肠道菌群的功能相似; 随着身体的成长和发育，肠道菌群的组成和功能也发生了变化。在幼虫发育的早期，肠道菌群的功能相似；随着身体的成长和发育，肠道菌群的组成和功能也发生了变化。在幼虫发育的早期，肠道菌群的功能相似；随着身体的成长和发育，肠道菌群的组成和功能也发生了变化。