Simple and Label-Free Liquid-Crystal-Based Detection of Acetylcholinesterase through Interactions between Liquid Crystals and Oil-in-Water Emulsion Droplets,ChemistrySelect

当前位置： X-MOL 学术 › ChemistrySelect › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Simple and Label-Free Liquid-Crystal-Based Detection of Acetylcholinesterase through Interactions between Liquid Crystals and Oil-in-Water Emulsion Droplets
ChemistrySelect ( IF 1.9 ) Pub Date : 2021-09-13 , DOI: 10.1002/slct.202100536
Dong Yoon Jeon ₁ , Chang‐Hyun Jang ₂

Affiliation

Here, we report a liquid crystal (LC)-based sensing method for the detection of acetylcholinesterase (AChE) and its inhibitor. The LC and glyceryl trioleate (GT)-in-water emulsion droplets were decorated with myristoylcholine chloride (Myr). The decoration helps to prevent the coalescence between the LC and GT emulsion droplets and therefore keeps the LCs in the nematic state, resulting in a bright optical image. In the presence of AChE, Myr was hydrolyzed, causing coalescence of LC and GT emulsion droplets, inducing the phase transition of LCs from the nematic to the isotropic state, resulting in a dark optical image. The biosensor exhibited a limit of detection (LOD) of 100 ng/mL. Moreover, the biosensor was highly specific for AChE and showed a high sensitivity for the detection of AChE inhibitor, with a LOD of 10 μg/mL. The biosensor is a simple and convenient sensing platform for the detection of AChE and its inhibitors.

中文翻译：

通过液晶和水包油乳液液滴之间的相互作用，简单且无标记的基于液晶的乙酰胆碱酯酶检测

在这里，我们报告了一种基于液晶 (LC) 的传感方法，用于检测乙酰胆碱酯酶 (AChE) 及其抑制剂。LC 和三油酸甘油酯 (GT)-水乳剂液滴用氯化肉豆蔻酰胆碱 (Myr) 装饰。装饰有助于防止 LC 和 GT 乳剂液滴之间的聚结，从而使 LC 保持向列状态，从而产生明亮的光学图像。在 AChE 存在下，Myr 被水解，导致 LC 和 GT 乳液液滴的聚结，诱导 LC 从向列状态到各向同性状态的相变，从而产生暗光学图像。该生物传感器的检测限 (LOD) 为 100 ng/mL。此外，该生物传感器对 AChE 具有高度特异性，对 AChE 抑制剂的检测显示出高灵敏度，LOD 为 10 μg/mL。

更新日期：2021-09-13

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11