A 56-day feeding trial was conducted to evaluate the effects of replacing fish meal (FM) with low-gossypol cottonseed meal (LCSM) on liver immune of silver sillago Sillago sihama Forsskál (1775). Five isonitrogenous and isolipidic diets were formulated with 0%, 16%, 32%, 48% and 64% LCSM replacing FM level (supplemented with methionine and lysine, named LCSM0, LCSM160, LCSM320, LCSM480 and LCSM640). Growth indices were significantly higher in the LCSM0 and LCSM160 groups than in the LCSM480 and LCSM640 groups. Malondialdehyde (MDA) content, alkaline phosphatase (AKP) activity and reactive oxygen species (ROS) concentrations in the liver of the control group were higher than those of the treatment group. Liver catalase (CAT), glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), lysozyme (LYZ) activity, immunoglobulin M (IgM) levels and total antioxidative capacity (T-AOC) levels were highest in LCSM320 group. Dietary LCSM substitution up-regulated the expression of liver interleukin 10 (IL-10) and downregulated tumour necrosis factor-α (TNF-α), target of rapamycin (TOR), insulin-like growth factor 1 (IGF-1) and transforming growth factor beta-3 (TGF-β3) expression. The relative mRNA expression level in liver nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and interleukin one beta (IL-1β) was not affected by dietary LCSM levels. The fish meal replacement level reached 32% and some genes in the complement pathway were significantly up-regulated (p < .05) including complement component 3 (C3), complement factor H (CFH), complement factor B (CFB) and MBL-associated serine protease 1 (MASP1). These results show that 16% of the fish meal replaced by LCSM with amino acids (methionine and lysine) supplementation did not significantly reduce immunity or antioxidant parameter compared with the FM-based control.

中文翻译：

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以低棉酚棉籽粕代替鱼粉的日粮饲喂silver sillago、Sillago sihama的肝脏免疫参数、补体途径、炎症因子和TOR基因表达

进行了为期 56 天的喂养试验，以评估用低棉酚棉籽粉 (LCSM) 替代鱼粉 (FM) 对银西拉西拉西哈马肝脏免疫的影响福斯卡尔 (1775)。用 0%、16%、32%、48% 和 64% LCSM 替代 FM 水平（补充蛋氨酸和赖氨酸，命名为 LCSM0、LCSM160、LCSM320、LCSM480 和 LCSM640）配制了五种等氮和等脂饮食。LCSM0 和 LCSM160 组的生长指数显着高于 LCSM480 和 LCSM640 组。对照组肝脏丙二醛(MDA)含量、碱性磷酸酶(AKP)活性和活性氧(ROS)浓度均高于治疗组。LCSM320组肝过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、总超氧化物歧化酶（T-SOD）、溶菌酶（LYZ）活性、免疫球蛋白M（IgM）水平和总抗氧化能力（T-AOC）水平最高. 膳食 LCSM 替代上调肝白细胞介素 10 (IL-10) 的表达并下调肿瘤坏死因子-α (TNF-α)、雷帕霉素靶点 (TOR)、胰岛素样生长因子 1 (IGF-1) 和转化生长因子 β-3 (TGF-β3) 的表达。活化 B 细胞 (NF-κB) 和白细胞介素一β (IL-1β) 的肝核因子 kappa-轻链增强子中的相对 mRNA 表达水平不受膳食 LCSM 水平的影响。鱼粉替代水平达到32%，补体途径部分基因显着上调。活化 B 细胞 (NF-κB) 和白细胞介素一β (IL-1β) 的肝核因子 kappa-轻链增强子中的相对 mRNA 表达水平不受膳食 LCSM 水平的影响。鱼粉替代水平达到32%，补体途径部分基因显着上调。活化 B 细胞 (NF-κB) 和白细胞介素一β (IL-1β) 的肝核因子 kappa-轻链增强子中的相对 mRNA 表达水平不受膳食 LCSM 水平的影响。鱼粉替代水平达到32%，补体途径部分基因显着上调。p  < .05) 包括补体成分 3 (C3)、补体因子 H (CFH)、补体因子 B (CFB) 和 MBL 相关丝氨酸蛋白酶 1 (MASP1)。这些结果表明，与基于 FM 的对照相比，用补充氨基酸（蛋氨酸和赖氨酸）的 LCSM 替代 16% 的鱼粉并未显着降低免疫力或抗氧化参数。