Microbubble Functionalization with Platelet Membrane Enables Targeting and Early Detection of Sepsis-Induced Acute Kidney Injury,Advanced Healthcare Materials

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Microbubble Functionalization with Platelet Membrane Enables Targeting and Early Detection of Sepsis-Induced Acute Kidney Injury
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2021-09-12 , DOI: 10.1002/adhm.202101628
Jing Yang ₁ , Xiaoyan Miao ₂ , Yu Guan ₁ , Chaojin Chen ₁ , Sufang Chen ₁ , Xinmin Zhang ₃ , Xue Xiao ₁ , Zheng Zhang ₁ , Zhengyuan Xia ₄ , Tinghui Yin ₂ , Ziqing Hei ₁ , Weifeng Yao ₁

Affiliation

The morbidity and mortality of sepsis-induced acute kidney injury (SAKI) remain high. Early detection using molecular ultrasound imaging may reduce mortality and improve the prognosis. Inspired by the intrinsic relationship between platelets and SAKI, platelet membrane-coated hybrid microbubbles (Pla-MBs) are designed for early recognition of SAKI. Pla-MBs are prepared by ultrasound-assisted recombination of liposomes and platelets, consisting of inherent platelet membrane isolated from platelets. By coating with platelet membranes, Pla-MBs are endowed with various adhesive receptors (such as integrin αIIbβ3), providing a benefit for selective adhesion to damaged endothelium in SAKI. In a rat SAKI model, by combining the advantages of molecular ultrasound imaging and platelet membrane, Pla-MBs display platelet-mimicking properties and achieve the early targeted diagnosis of SAKI prior to the regular laboratory markers of kidney function. Moreover, the expression of platelet-binding proteins (von Willebrand factor and fibrinogen) in the kidneys shows consistent results with molecular ultrasound imaging. Together, microbubble functionalization with platelet membranes is diagnostically beneficial for SAKI and might be a promising modality for endothelial injury diseases in the future.

中文翻译：

血小板膜的微泡功能化能够靶向和早期检测脓毒症引起的急性肾损伤

脓毒症引起的急性肾损伤 (SAKI) 的发病率和死亡率仍然很高。使用分子超声成像进行早期检测可以降低死亡率并改善预后。受血小板和 SAKI 之间内在关系的启发，血小板膜包覆的混合微泡 (Pla-MBs) 被设计用于早期识别 SAKI。Pla-MB 是通过脂质体和血小板的超声辅助重组制备的，由从血小板中分离出来的固有血小板膜组成。通过用血小板膜包被，Pla-MBs 被赋予了各种粘附受体（如整合素α IIb β3），为SAKI中受损内皮的选择性粘附提供了益处。在大鼠SAKI模型中，通过结合分子超声成像和血小板膜的优势，Pla-MBs表现出血小板模拟特性，在常规的肾功能实验室指标之前实现了SAKI的早期靶向诊断。此外，肾脏中血小板结合蛋白（血管性血友病因子和纤维蛋白原）的表达与分子超声成像结果一致。总之，具有血小板膜的微泡功能化在诊断上对 SAKI 是有益的，并且可能是未来内皮损伤疾病的一种有前途的方式。

更新日期：2021-09-12

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