Microalgae cultivation for exopolysaccharide production has getting more attention as a result of their high hydrocarbon biosynthesis skill. The aim of this study is to examine the exopolysaccharide production potential of different species of microalgae. In this context, exopolysaccharides were produced from Chlorella minutissima, Chlorella sorokiniana and Botryococcus braunii microalgae and the effects of carbon and nitrogen content in the growth medium and illumination time on exopolysaccharide production were analyzed statistically using Box-Behnken experimental design. In addition, techno-economic assessment of exopolysaccharide production were also performed by using the most productive microalgae and optimum conditions determined in this study. As a result of the experiments, it was seen that C. minutissima, C. sorokiniana and B. braunii produced 0.245 ± 0.0025 g/L, 0.163 ± 0.0016 g/L and 0.117 ± 0.0007 g/L exopolysaccharide, respectively. Statistically, it was observed that there was an inverse relationship between the exopolysaccharide production and investigated parameters such as illumination period and carbon and nitrogen amounts of culture mediums. The techno-economic assessment comprising microalgal exopolysaccharide (EPS) bioprocess was carried out, and it showed that the system can be considered economically viable, yet can be improved with biorefinery approach.

用于生产胞外多糖的微藻培养由于其高烃生物合成能力而受到越来越多的关注。本研究的目的是检验不同种类微藻的胞外多糖生产潜力。在这种情况下，胞外多糖是由小球藻、小球藻和Botryococcus braunii生产的使用 Box-Behnken 实验设计对微藻以及生长培养基中碳和氮含量以及光照时间对胞外多糖生产的影响进行了统计分析。此外，还通过使用本研究中确定的最高产微藻和最佳条件对胞外多糖生产进行了技术经济评估。实验结果表明，C. minutissima、C. sorokiniana和B. braunii分别产生 0.245 ± 0.0025 g/L、0.163 ± 0.0016 g/L 和 0.117 ± 0.0007 g/L 胞外多糖。统计上，观察到胞外多糖的产生与研究的参数（例如光照时间和培养基的碳和氮量）之间存在反比关系。进行了包括微藻胞外多糖 (EPS) 生物工艺的技术经济评估，结果表明该系统在经济上是可行的，但可以通过生物精炼方法进行改进。