ABSTRACT

Artemisia rupestris L. polysaccharides have immune enhancement effects with low toxicity in the previous studies. The effect of crude polysaccharides from cultivated Artemisia rupestris L. (CPCAR) on immunity has not been explored. Here, the adjuvant potential of CPCAR was investigated in vivo and its underlying mechanism in vitro was explored through activation of bone marrow-derived dendritic cells (BMDCs). After intramuscular immunizations, CPCAR elevated IgG levels with a T_H1-biased response, lymphocyte proliferation and increased CD4 and CD8 T cells in spleen and lymph nodes. IFN-γ and IL-4 of CD8 or CD4 T cells were specifically increased. CPCAR improved DC maturation and diminished Treg frequency. BMDCs treated with CPCAR in vitro showed the up-regulation of CD40, CD86, IL-12 and TNF-α. BMDMs pretreated with TLR4 and NF-kB inhibitors decreased the expressions of CD40 and CD86 and produced less cytokines. These data demonstrated that CPCAR could boost T_H1-biased response through DC activation in TLR4/NF-κB signalling.

摘要

在以往的研究中，青蒿多糖具有免疫增强作用，毒性低。尚未探索来自栽培蒿(CPCAR)的粗多糖对免疫的影响。在这里，在体内研究了 CPCAR 的佐剂潜力，并通过激活骨髓衍生树突细胞 (BMDC) 探索了其体外潜在机制。肌内免疫后，CPCAR 升高 IgG 水平，T _H1 偏向反应、淋巴细胞增殖以及脾脏和淋巴结中 CD4 和 CD8 T 细胞增加。CD8 或 CD4 T 细胞的 IFN-γ 和 IL-4 特异性增加。CPCAR 改善 DC 成熟并降低 Treg 频率。体外CPCAR 处理的 BMDCs显示 CD40、CD86、IL-12 和 TNF-α 的上调。用 TLR4 和 NF-kB 抑制剂预处理的 BMDM 降低了 CD40 和 CD86 的表达并产生较少的细胞因子。这些数据表明，CPCAR 可以通过 TLR4/NF-κB 信号传导中的 DC 激活来增强 T _H 1 偏向反应。