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Improving the photostability of fluorescent dyes by polymer nano-insulating layer
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2021-09-13 , DOI: 10.1002/app.51625 Yingshi Zhao 1 , Qinyong Song 1 , Yuebin Lin 1 , Feng Chu 2 , Yanchun Wei 1 , Sen Liu 1 , Changjiang Pan 1 , Li Quan 1 , Yiqing Wang 2
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2021-09-13 , DOI: 10.1002/app.51625 Yingshi Zhao 1 , Qinyong Song 1 , Yuebin Lin 1 , Feng Chu 2 , Yanchun Wei 1 , Sen Liu 1 , Changjiang Pan 1 , Li Quan 1 , Yiqing Wang 2
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Fluorescein is a traditional organic fluorescent dye that has the disadvantage of agglomeration-induced fluorescence quenching and photobleaching due to its large hydrophobic conjugate π-π system, which makes them have limitations in many fields of application. A method of polymer modification is revealed to address this issue. In this study, fluorescein molecules were modified by polycaprolactone (PCL) to obtain PCL grafting fluorescein (PxF), and subsequently assembled with PEG-PCL to obtain fluorescent nanoparticles (PPxF). PPxF would realize long term cellular fluorescence imaging. PCL polymerization induces self-assembly, which effectively avoids fluorescence quenching caused by fluorescent molecules aggregation, and at the same time well inhibit the energy transfer with oxygen to improve photostability. This method is a good way to improve the fluorescence properties of fluorescent dyes, to be suitable for all traditional organic fluorescent dyes to greatly improve their fluorescence and hydrophilic properties and enhance their application fields.
中文翻译：
聚合物纳米绝缘层提高荧光染料的光稳定性
荧光素是一种传统的有机荧光染料，由于其庞大的疏水共轭π-π体系，存在团聚诱导荧光猝灭和光漂白的缺点，这使得它们在许多应用领域受到限制。揭示了聚合物改性的方法来解决这个问题。本研究通过聚己内酯（PCL）修饰荧光素分子，获得PCL接枝荧光素（P xF)，随后与 PEG-PCL 组装以获得荧光纳米粒子 (PPxF)。PPxF 将实现长期细胞荧光成像。PCL聚合诱导自组装，有效避免了荧光分子聚集引起的荧光猝灭，同时很好地抑制了与氧的能量转移，提高了光稳定性。该方法是提高荧光染料荧光性能的好方法，适用于所有传统的有机荧光染料，大大提高其荧光和亲水性能，扩大其应用领域。
更新日期：2021-11-01
中文翻译：
聚合物纳米绝缘层提高荧光染料的光稳定性
荧光素是一种传统的有机荧光染料，由于其庞大的疏水共轭π-π体系，存在团聚诱导荧光猝灭和光漂白的缺点，这使得它们在许多应用领域受到限制。揭示了聚合物改性的方法来解决这个问题。本研究通过聚己内酯（PCL）修饰荧光素分子，获得PCL接枝荧光素（P xF)，随后与 PEG-PCL 组装以获得荧光纳米粒子 (PPxF)。PPxF 将实现长期细胞荧光成像。PCL聚合诱导自组装，有效避免了荧光分子聚集引起的荧光猝灭，同时很好地抑制了与氧的能量转移，提高了光稳定性。该方法是提高荧光染料荧光性能的好方法，适用于所有传统的有机荧光染料，大大提高其荧光和亲水性能，扩大其应用领域。
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