During its first hours of development, the zebrafish embryo presents a large microtubule array in the yolk region, essential for its development. Despite of its size and dynamic behavior, this network has been studied only in limited field of views or in fixed samples. We designed and implemented different strategies in Light Sheet Fluorescence microscopy for imaging the entire yolk microtubule (MT) network in vivo. These have allowed us to develop a novel image analysis from which we clearly observe a cyclical re-arrangement of the entire MT network in synchrony with blastoderm mitotic waves. These dynamics also affect a previously unreported microtubule array deep within the yolk, here described. These findings provide a new vision of the zebrafish yolk microtubules arrangement, and offers novel insights in the interaction between mitotic events and microtubules reorganization.

中文翻译：

---

用于斑马鱼胚胎微管动力学体内研究的光片荧光显微镜

在发育的最初几个小时，斑马鱼胚胎在卵黄区域呈现出一个大的微管阵列，这对其发育至关重要。尽管它的大小和动态行为，该网络仅在有限的视野或固定样本中进行了研究。我们在光片荧光显微镜中设计并实施了不同的策略，用于在体内对整个蛋黄微管 (MT) 网络进行成像. 这些使我们能够开发一种新颖的图像分析，从中我们可以清楚地观察到整个 MT 网络与胚盘有丝分裂波同步的周期性重新排列。这些动态也影响了以前未报告的蛋黄深处的微管阵列，这里描述。这些发现提供了斑马鱼卵黄微管排列的新视野，并为有丝分裂事件和微管重组之间的相互作用提供了新的见解。