Microalgae are coming to the spotlight due to their potential applications in a wide number of fields ranging from the biofuel to the pharmaceutical sector. However, several factors such as low productivity, expensive harvesting procedures and difficult metabolite extractability limit their full utilization at industrial scale. Similarly to the successful employment of enzymatic arsenals from lignocellulolytic fungi to convert lignocellulose into fermentable sugars for bioethanol production, specific algalytic formulations could be used to improve the extractability of lipids from microalgae to produce biodiesel. Currently, the research areas related to algivorous organisms, algal saprophytes and the enzymes responsible for the hydrolysis of algal cell wall are still little explored. Here, an algal trap method for capturing actively growing microorganisms was successfully used to isolate a filamentous fungus, that was identified by whole-genome sequencing, assembly and annotation as a novel Penicillium sumatraense isolate. The fungus, classified as P. sumatraense AQ67100, was able to assimilate heat-killed Chlorella vulgaris cells by an enzymatic arsenal composed of proteases such as dipeptidyl- and amino-peptidases, β-1,3-glucanases and glycosidases including α- and β-glucosidases, β-glucuronidase, α-mannosidases and β-galactosidases. The treatment of C. vulgaris with the filtrate from P. sumatraense AQ67100 increased the release of chlorophylls and lipids from the algal cells by 42.6 and 48.9%, respectively. The improved lipid extractability from C. vulgaris biomass treated with the fungal filtrate highlighted the potential of algal saprophytes in the bioprocessing of microalgae, posing the basis for the sustainable transformation of algal metabolites into biofuel-related compounds.

中文翻译：

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一种新型的苏门答腊青霉分离株揭示了藻类生物精炼过程中可利用的降解酶库

微藻因其在从生物燃料到制药领域的广泛领域的潜在应用而成为人们关注的焦点。然而，诸如低生产力、昂贵的收获程序和难以提取的代谢物等几个因素限制了它们在工业规模上的充分利用。与成功使用来自木质纤维素分解真菌的酶库将木质纤维素转化为用于生物乙醇生产的可发酵糖类类似，特定的海藻制剂可用于提高微藻脂质的可提取性，以生产生物柴油。目前，与藻类生物、藻类腐生植物和负责藻类细胞壁水解的酶相关的研究领域还鲜有探索。这里，用于捕获活跃生长的微生物的藻类捕获方法已成功用于分离丝状真菌，通过全基因组测序、组装和注释将其鉴定为新型苏门答腊青霉分离株。该真菌被归类为 P. sumatraense AQ67100，能够通过由蛋白酶（如二肽基和氨基肽酶、β-1,3-葡聚糖酶和糖苷酶（包括 α-和β）组成的酶库吸收热杀死的普通小球藻细胞） -葡萄糖苷酶、β-葡萄糖醛酸酶、α-甘露糖苷酶和β-半乳糖苷酶。用来自 P. sumatraense AQ67100 的滤液处理 C. vulgaris 使藻类细胞中叶绿素和脂质的释放分别增加了 42.6% 和 48.9%。提高了从 C.