6-propionyl-2-dimethylaminonaphthalene (PRODAN) is a fluorescent molecule with sensitivity to environmental polarity and hydrogen bond interactions. Although it has been extensively used to study organized systems such as vesicles and reverse micelles, its potential application to characterize polymeric micelles remains largely unexplored. Molecular probes can provide valuable information about the physicochemical properties of these micelles, and thus about the environment they offer to solubilize cargos. The present study aimed to find out what new insights could be gained by using PRODAN for such a purpose. The model systems chosen were D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) and polyvinyl caprolactam-polyvinyl acetate-poly-ethylene glycol (Soluplus®) micelles. Steady-state and time-resolved fluorescence emission spectroscopy revealed that PRODAN was wholly incorporated into the micelles when the polymer concentration exceeded 9.9x10^-4 M (TGPS) and 2.1x10^-5 M (Soluplus®). The probe sensed a less polar environment inside Soluplus® micelles, whose core was found to be more fluid than that of TGPS micelles through red edge excitation shift (REES) experiments. A calculation of the well-known polarity parameter E_T(30) confirmed that the environment was less polar within Soluplus® micelles than within TGPS ones. These results correlate well with the known loading capacity of both systems to solubilize different drugs, and therefore demonstrate that PRODAN is a powerful probe to characterize polymeric micelles.

6-丙酰基-2-二甲氨基萘 (PRODAN) 是一种对环境极性和氢键相互作用敏感的荧光分子。尽管它已被广泛用于研究有组织的系统，如囊泡和反胶束，但其在表征聚合物胶束方面的潜在应用仍然在很大程度上未被探索。分子探针可以提供关于这些胶束的物理化学特性的有价值的信息，从而提供关于它们为溶解货物提供的环境的信息。本研究旨在找出为此目的使用 PRODAN 可以获得哪些新见解。选择的模型系统是 D-α-生育酚聚乙二醇 1000 琥珀酸酯 (TPGS) 和聚乙烯基己内酰胺-聚乙酸乙烯酯-聚乙二醇 (Soluplus®) 胶束。^-4 M (TGPS) 和 2.1x10 ^-5 M (Soluplus®)。探针检测到 Soluplus® 胶束内部的极性较小的环境，通过红边激发偏移 (REES) 实验发现其核心比 TGPS 胶束的核心更具流动性。众所周知的极性参数 E _T (30) 的计算证实，Soluplus® 胶束内的环境极性低于 TGPS 胶束内的极性。这些结果与两种系统溶解不同药物的已知负载能力密切相关，因此证明 PRODAN 是表征聚合物胶束的强大探针。