GhADF6-mediated actin reorganization is associated with defence against Verticillium dahliae infection in cotton,Molecular Plant Pathology

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GhADF6-mediated actin reorganization is associated with defence against Verticillium dahliae infection in cotton
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-09-13 , DOI: 10.1111/mpp.13137
Yongduo Sun _{1,

2,

3} , Mengmeng Zhong _{1,

2,

3} , Yuanbao Li _{1,

2,

3} , Ruihui Zhang _{3,

4} , Lei Su _{1,

2} , Guixian Xia _{1,

2} , Haiyun Wang _{1,

2}

Affiliation

Several studies have revealed that actin depolymerizing factors (ADFs) participate in plant defence responses; however, the functional mechanisms appear intricate and need further exploration. In this study, we identified an ADF6 gene in upland cotton (designated as GhADF6) that is evidently involved in cotton's response to the fungal pathogen Verticillium dahliae. GhADF6 binds to actin filaments and possesses actin severing and depolymerizing activities in vitro and in vivo. When cotton root (the site of the fungus invasion) was inoculated with the pathogen, the expression of GhADF6 was markedly down-regulated in the epidermal cells. By virus-induced gene silencing analysis, the down-regulation of GhADF6 expression rendered the cotton plants tolerant to V. dahliae infection. Accordingly, the abundance of actin filaments and bundles in the root cells was significantly higher than that in the control plant, which phenocopied that of the V. dahliae-challenged wild-type cotton plant. Altogether, our results provide evidence that an increase in filament actin (F-actin) abundance as well as dynamic actin remodelling are required for plant defence against the invading pathogen, which are likely to be fulfilled by the coordinated expressional regulation of the actin-binding proteins, including ADF.

中文翻译：

GhADF6介导的肌动蛋白重组与棉花对黄萎病菌感染的防御有关

几项研究表明，肌动蛋白解聚因子 (ADF) 参与植物防御反应。然而，功能机制似乎错综复杂，需要进一步探索。在这项研究中，我们在陆地棉中发现了一个ADF6基因（称为GhADF6），该基因明显参与了棉花对真菌病原体黄萎病菌的反应。GhADF6 与肌动蛋白丝结合并在体外和体内具有肌动蛋白切断和解聚活性。当棉花根部（真菌入侵部位）接种病原菌后，表皮细胞中GhADF6的表达明显下调。通过病毒诱导的基因沉默分析，GhADF6的下调表达使棉花植物对V. dahliae感染具有耐受性。因此，根细胞中肌动蛋白丝和束的丰度显着高于对照植物，这与大丽花挑战的野生型棉花植物的表型相似。总而言之，我们的结果提供了证据表明，植物防御入侵病原体需要增加丝状肌动蛋白（F-肌动蛋白）丰度以及动态肌动蛋白重塑，这可能通过肌动蛋白结合的协调表达调控来实现蛋白质，包括 ADF。

更新日期：2021-11-10

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