We investigated whether standardized edible bird’s nest extract (BNE-PK) can prevent ultraviolet B (UVB) irradiation-mediated oxidative stress and photoaging in the skin using in vitro and in vivo models. BNE-PK increased skin hydration by hyaluronic acid synthesis and activation of ceramide synthase in UVB-irradiated hairless mice and HaCaT cells. Furthermore, BNE-PK suppressed melanogenesis by down-regulation of the cAMP/PKA/CREB/MITF/TRP-1/TRP-2/tyrosinase pathway in UVB-irradiated hairless mice and 3-isobutyl-1-methylxanthine (IBMX)-treated B16F10 cells. In UVB-irradiated hairless mice, BNE-PK attenuated the wrinkle formation-related JNK/c-FOS/c-Jun/MMP pathway and activated the TGF-βRI/SMAD3/pro-collagen type I pathway during UVB-mediated oxidative stress. Based on these findings, our data suggest that BNE-PK may potentially be used for the development of effective natural anti-photoaging functional foods for skin health.

中文翻译：

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标准化食用燕窝提取物可防止皮肤中 UVB 辐射介导的氧化应激和光老化

我们使用体外和体内模型研究了标准化食用燕窝提取物 (BNE-PK) 是否可以防止紫外线 B (UVB) 辐射介导的皮肤氧化应激和光老化。BNE-PK 通过透明质酸合成和激活 UVB 照射的无毛小鼠和 HaCaT 细胞中的神经酰胺合酶来增加皮肤水合作用。此外，BNE-PK 通过下调 UVB 照射的无毛小鼠和 3-异丁基-1-甲基黄嘌呤 (IBMX) 处理的 cAMP/PKA/CREB/MITF/TRP-1/TRP-2/酪氨酸酶途径抑制黑色素生成B16F10 细胞。在 UVB 照射的无毛小鼠中，BNE-PK 减弱了与皱纹形成相关的 JNK/c-FOS/c-Jun/MMP 通路，并在 UVB 介导的氧化应激期间激活了 TGF-βRI/SMAD3/pro-collagen I 型通路。基于这些发现，