Our goal was to validate a human hyaluronic acid (HA) ELISA (Hyaluronic acid plus ELISA; TECOmedical Group) for use in feline plasma. Plasma from 5 healthy cats and 5 critically ill cats was used for validation of the assay. Validation methods performed included intra- and inter-assay variability, spike-and-recovery, and dilutional linearity. All measurements were performed in duplicate. The precision study revealed good intra-assay CV of 7.4–8.9%; inter-assay CV was 3.4–4.2%. Extraction efficiency via spiking tests yielded mean recovery of 89.6%. The assay met criteria for acceptable linearity using 3 serial dilutions. Our results demonstrate that this commercial HA ELISA had acceptable analytical performance using feline plasma and could be a useful tool in the veterinary clinical research setting.

我们的目标是验证用于猫血浆的人透明质酸 (HA) ELISA（透明质酸加 ELISA；TECOmedical Group）。来自 5 只健康猫和 5 只重病猫的血浆用于验证该测定。执行的验证方法包括测定内和测定间变异性、加标和回收率以及稀释线性。所有测量均重复进行。精密度研究显示，7.4-8.9% 的测定内 CV 良好；测定间 CV 为 3.4–4.2%。通过加标测试的提取效率产生了 89.6% 的平均回收率。使用 3 次连续稀释，该测定符合可接受的线性标准。我们的结果表明，这种商业 HA ELISA 使用猫血浆具有可接受的分析性能，并且可以成为兽医临床研究环境中的有用工具。