Nucleosides can be used as quality evaluation indicators of Tricholoma matsutake. In this work, a new ultra-performance liquid chromatography-tandem mass spectrometry (UPLC/MS) strategy for quantitative analysis of multiple components using a single marker (QAMS) was proposed to determine nine nucleosides (adenosine, cytidine, guanosine, inosine, uridine, 2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine, and 2′-deoxyuridine) in T. matsutake. Guanosine was set as the internal reference substance, whose content in T. matsutake was determined using the conventional external standard method. Relative correction factors (RCFs) between guanosine and eight other nucleosides were measured. The concentrations of the eight components were calculated with the obtained RCFs by QAMS. An ultrasonic extraction method is used for sample preparation. This method was validated to be sensitive, precise, and accurate with the LODs of 0.31–1.9 ng, the overall intraday and interday variations less than 4.08%, and the overall recovery over 89.0%. The correlation coefficients (r²) of the calibration curves were higher than 0.9918. The values of vector angle analysis were above 0.99845, which indicates no significant differences between the conventional external standard method and the present QAMS method. As far as we know, this is also the first report of UPLC/MS analysis of nucleoside compounds by QAMS, providing an efficient and feasible quality assessment method for other natural products containing nucleosides.

中文翻译：

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UPLC/MS 测定松茸中九种核苷的实用方法及单标记法多组分定量分析

核苷可作为松茸质量评价指标。在这项工作中，提出了一种使用单一标记物 (QAMS) 对多种成分进行定量分析的新型超高效液相色谱-串联质谱 (UPLC/MS) 策略，以确定九种核苷（腺苷、胞苷、鸟苷、肌苷、尿苷）。 、2'-脱氧腺苷、2'-脱氧胞苷、2'-脱氧鸟苷和2'-脱氧尿苷）在松茸中。以鸟苷为内参，其在松茸中的含量采用常规外标法测定。测量了鸟苷和其他八种核苷之间的相对校正因子 (RCF)。八种成分的浓度通过 QAMS 用获得的 RCF 计算。超声波提取方法用于样品制备。该方法经验证灵敏、精确和准确，LOD 为 0.31–1.9 ng，整体日内和日间变化小于 4.08%，总回收率超过 89.0%。相关系数 ( r ²) 的校准曲线高于 0.9918。矢量角分析值均在 0.99845 以上，表明常规外标法与现用 QAMS 法无显着差异。据我们所知，这也是第一篇用QAMS对核苷化合物进行UPLC/MS分析的报道，为其他含核苷的天然产物提供了一种高效可行的质量评估方法。