Three-dimensional (3D) bioprinting is a revolutionary technology that replicates 3D functional living tissue scaffolds in vitro by controlling the layer-by-layer deposition of biomaterials and enables highly precise positioning of cells. With the development of this technology, more advanced research on the mechanisms of tissue morphogenesis, clinical drug screening, and organ regeneration may be pursued. Because of their self-renewal characteristics and multidirectional differentiation potential, induced pluripotent stem cells (iPSCs) have outstanding advantages in stem cell research and applications. In this review, we discuss the advantages of different bioinks containing human iPSCs that are fabricated by using 3D bioprinting. In particular, we focus on the ability of these bioinks to support iPSCs and promote their proliferation and differentiation. In addition, we summarize the applications of 3D bioprinting with iPSC-containing bioinks and put forward new views on the current research status.

中文翻译：

---

支持诱导多能干细胞的3D生物打印支架的开发与应用

三维 (3D) 生物打印是一项革命性的技术，它通过控制生物材料的逐层沉积，在体外复制 3D 功能性活组织支架，并实现细胞的高精度定位。随着该技术的发展，可能会在组织形态发生、临床药物筛选和器官再生的机制方面进行更深入的研究。诱导多能干细胞（iPSCs）由于具有自我更新特性和多向分化潜能，在干细胞研究和应用方面具有突出的优势。在这篇综述中，我们讨论了使用 3D 生物打印制造的含有人类 iPSC 的不同生物墨水的优势。特别是，我们专注于这些生物墨水支持 iPSC 并促进其增殖和分化的能力。此外，我们总结了含 iPSC 的生物墨水在 3D 生物打印中的应用，并对目前的研究现状提出了新的看法。