To gain a high yield of xylooligosaccharides (XOS) at a low cost, W/W droplet-based microfluidic system combined with immobilized xylanase was constructed. Jetting-regime under the high viscosity of both two phases was correlated with the Ca and We numbers. W/W droplets were obtained with controllable sizes when the flow velocities of dispersed and continuous phases were in the range of 0.29–0.48 µL/min and 0.52–0.80 µL/min respectively. The droplet size could be expressed as a power-law relationship with the velocity ratio of two phases. The maximum yield of XOS was 75.0% within 10 min. K_m value was reduced to a half compared with batch reactors. Xylanase-polymer conjugates were reused for 10 times, with a residual activity over 60%. The results confirmed that the combination of enzyme-polymer conjugates and W/W droplet-based microfluidic system is a low-cost and high catalytic efficiency method for biocatalysis.

为了以低成本获得高产量的低聚木糖 (XOS)，构建了结合固定化木聚糖酶的 W/W 液滴微流体系统。两相高粘度下的喷射方式与Ca和We数相关。当分散相和连续相的流速分别在 0.29–0.48 µL/min 和 0.52–0.80 µL/min 范围内时，获得的 W/W 液滴具有可控尺寸。液滴尺寸可以表示为与两相速度比的幂律关系。XOS 在 10 分钟内的最大产率为 75.0%。ķ_米与间歇式反应器相比，该值减少了一半。木聚糖酶-聚合物偶联物重复使用 10 次，残留活性超过 60%。结果证实，酶-聚合物偶联物和基于 W/W 液滴的微流体系统的组合是一种低成本、高催化效率的生物催化方法。