Abstract

Phyllanthus emblica L. is a widely distributed tropical medicinal plant with good therapeutic properties. In the present study, the chemical constituents isolation of the roots of P. emblica were carried out and six known compounds (1-6) were purified and their structures were determined by means of spectroscopic analysis. The known triterpenoid, secofriedelanophyllemblicine (1), was selected to check for its cytotoxic effect on a series of human tumor cells and normal cells. Secofriedelanophyllemblicine exhibited cell growth inhibition specifically on MCF-7 breast cancer cells when compared to other tested cell lines. Further flow cytometric analysis showed that secofriedelanophyllemblicine could inhibit cell proliferation and induced G2 phase arrest in the cell-cycle progression of MCF-7 cells. The gathered results suggest that secofriedelanophyllemblicine is a cell-cycle regulator in MCF-7 human breast cancer cells and might be used as a candidate chemopreventive agent for breast cancer prevention and intervention.

摘要

余甘子是一种分布广泛的热带药用植物，具有良好的治疗作用。本研究对余甘子根的化学成分进行了分离，纯化了6种已知化合物( 1-6 )，并通过光谱分析确定了它们的结构。已知的三萜类化合物，secofriedelanophyllemblicine ( 1)，被选中来检查其对一系列人类肿瘤细胞和正常细胞的细胞毒性作用。与其他测试的细胞系相比，Secofriedelanophyllemblicine 对 MCF-7 乳腺癌细胞表现出特异性的细胞生长抑制作用。进一步的流式细胞术分析表明，secofriedelanophylemblicine 可以抑制细胞增殖并诱导 MCF-7 细胞周期进程的 G2 期停滞。收集的结果表明，secofriedelanophyllemblicine 是 MCF-7 人乳腺癌细胞中的细胞周期调节剂，可用作乳腺癌预防和干预的候选化学预防剂。