Abstract

Phalaenopsis wilsonii Rolfe is a vulnerable wild moth orchid species with important horticultural value. The complete chloroplast genome sequence of P. wilsonii was generated by de novo assembly using whole genome next-generation sequencing to provide genomic data for further conservation genetics, phylogeny and molecular breeding in Phalaenopsis. The complete plastome of P. wilsonii is 145,096 bp in length, containing two inverted repeats (IR) regions (24,787 bp), a large single-copy (LSC) region (84,688 bp), and a small single-copy (SSC) region (10,834 bp). The chloroplast genome encoded 119 unique genes, including 73 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. The overall GC content of the whole genome is 36.9%. Phylogenetic analysis indicated P. wilsonii was closely related to P. lowii.

摘要

蝴蝶兰是一种脆弱的野生蝴蝶兰，具有重要的园艺价值。P. wilsonii的完整叶绿体基因组序列是使用全基因组下一代测序从头组装产生的，为蝴蝶兰的进一步保护遗传学、系统发育和分子育种提供基因组数据。P. wilsonii的完整塑性体长度为 145,096 bp，包含两个反向重复 (IR) 区域 (24,787 bp)、一个大单拷贝 (LSC) 区域 (84,688 bp) 和一个小单拷贝 (SSC) 区域 (10,834 bp)。叶绿体基因组编码了 119 个独特的基因，包括 73 个蛋白质编码基因、38 个 tRNA 基因和 8 个 rRNA 基因。全基因组的总GC含量为36.9%。系统发育分析表明P. wilsonii与P. lowii密切相关。