We designed and synthesized a novel copper (II) complex, [Cu(ambt)₂(cnbz)₂], using 2-amino-6-methoxybenzothiazole (ambt) and 2-chloro-5-nitrobenzoic acid (cnbz) as ligands. Single-crystal X-ray diffraction (XRD), IR (infrared) spectra along with elemental analysis were employed to characterize its structure. Single-crystal XRD analysis illustrated the copper (II) complex belonged to the triclinic crystal system, space group P-1 and formed mononuclear hexa-coordinated structure. Electronic absorption, fluorescence spectrum and viscosity analysis were employed to assess the binding properties of the complex with CT-DNA (calf thymus DNA), as well as HSA (human serum albumin). The calculated binding constants K_a were 5.89 × 10⁴ M⁻¹ and 3.31 × 10⁶ M⁻¹ for CT-DNA and HSA, respectively, implying that complex [Cu(ambt)₂(cnbz)₂] displays intercalative bindings with CT-DNA and HSA. It also statically quenched CT-DNA/HSA fluorescence. CCK-8 assay revealed that compared to cisplatin, the complex exerts greater anti-proliferative influence against HepG2, HeLa and A549 cell lines. Flow cytometry revealed that [Cu(ambt)₂(cnbz)₂] arrested the cycle of HepG2 cells at G0/G1 phase, inducing apoptosis / necrosis. The degree of apoptosis / necrosis and inhibition of cell cycle is dose-, as well as time-dependent.

我们设计并合成了一种新型铜 (II) 配合物 [Cu(ambt) ₂ (cnbz) ₂ ]，使用 2-氨基-6-甲氧基苯并噻唑 (ambt) 和 2-氯-5-硝基苯甲酸 (cnbz) 作为配体。采用单晶 X 射线衍射 (XRD)、IR (红外) 光谱以及元素分析来表征其结构。单晶XRD分析表明铜(II)配合物属于三斜晶系，空间群P-1，形成单核六配位结构。采用电子吸收、荧光光谱和粘度分析来评估复合物与 CT-DNA（小牛胸腺 DNA）以及 HSA（人血清白蛋白）的结合特性。计算的结合常数K _aCT-DNA 和 HSA 分别为 5.89 × 10 ⁴ M ^-1和 3.31 × 10 ⁶ M ^-1，这意味着复合物 [Cu(ambt) ₂ (cnbz) ₂ ] 显示出与 CT-DNA 和 HSA 的嵌入结合。它还静态猝灭 CT-DNA/HSA 荧光。CCK-8 测定显示，与顺铂相比，该复合物对 HepG2、HeLa 和 A549 细胞系具有更大的抗增殖影响。流式细胞术显示[Cu(ambt) ₂ (cnbz) ₂ ]在G0/G1期阻滞HepG2细胞的周期，诱导细胞凋亡/坏死。细胞凋亡/坏死和细胞周期抑制的程度是剂量和时间依赖性的。