Abstract

Super-paramagnetic iron oxide nanoparticles were synthesized by a co-precipitation method, which was further functionalized with chitosan (CS). The composite was investigated for binding with Nystatin (Nyst), a polyene polyhydroxy antifungal widely used in management of mucocutaneous fungal infections. The structure of the Fe₃O₄/CS/Nyst composite was characterized by different physicochemical techniques. Transmission electron microscope analysis revealed the Fe₃O₄/CS/Nyst composite with a mean diameter of ∼10–20 nm in a monodispersed spherical form. Fourier-transform infrared spectroscopy analysis confirmed the binding of CS on Fe₃O₄ NPs and also the loading of Nyst on the nanostructure. The UV–Vis absorption spectroscopy demonstrated the Nyst drug loading by 22% capacity. The antifungal activity of the Fe₃O₄/CS/Nyst was evaluated against Candida species and Aspergillus fumigatus, which demonstrated excellent activity with a minimum inhibitory concentration ranging from 2 to 16 μg/mL. The MTT assay was used to evaluate the toxicity of compounds which did not reveal any toxic effect on the adipose derived mesenchymal stem cells. Regarding remarkable antifungal activities and low toxicity of this magnetic nano-compound, it could be a good candidate for target therapy of mucocutaneous and local fungal infections.

摘要

通过共沉淀法合成超顺磁性氧化铁纳米粒子，并用壳聚糖（CS）进一步功能化。研究了复合材料与制霉菌素 (Nyst) 的结合，制霉菌素是一种广泛用于治疗皮肤粘膜真菌感染的多烯多羟基抗真菌剂。Fe ₃ O ₄ /CS/Nyst 复合材料的结构通过不同的物理化学技术表征。透射电子显微镜分析显示平均直径为~10-20 nm 的单分散球形Fe ₃ O ₄ /CS/Nyst 复合材料。傅里叶变换红外光谱分析证实了 CS 与 Fe ₃ O _{4 的结合}NPs 以及 Nyst 在纳米结构上的负载。UV-Vis 吸收光谱证明 Nyst 载药量为 22%。Fe ₃ O ₄ /CS/Nyst 对念珠菌属和烟曲霉的抗真菌活性进行了评估，显示出极好的活性，最小抑制浓度范围为 2 至 16 μg/mL。MTT测定用于评估化合物的毒性，该化合物未显示对脂肪来源的间充质干细胞有任何毒性作用。由于这种磁性纳米化合物具有显着的抗真菌活性和低毒性，因此它可能是皮肤粘膜和局部真菌感染的靶向治疗的良好候选者。