Small-Diameter PLCL/PCL Nanofiber Grafted TSF Vascular Scaffolds with a Double-Layer Structure for Vascular Tissue Engineering,Macromolecular Materials and Engineering

当前位置： X-MOL 学术 › Macromol. Mater. Eng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Small-Diameter PLCL/PCL Nanofiber Grafted TSF Vascular Scaffolds with a Double-Layer Structure for Vascular Tissue Engineering
Macromolecular Materials and Engineering ( IF 4.2 ) Pub Date : 2021-09-12 , DOI: 10.1002/mame.202100462
Weili Shao _{1,

2} , Chen Cui ₁ , Junpeng Xiong ₁ , Ling Wang ₁ , Xu Zhao ₁ , Yijun Hou ₁ , Shuzhen Chang ₁ , Ning Sun ₁ , Yuting Zhang ₁ , Yanfei Gao _{1,

2} , Qingqing Ni ₃ , Fan Liu _{1,

2} , Jianxin He _{1,

2}

Affiliation

Successful construction of small-diameter double-layer vascular scaffolds (SDVSs) whose inner diameters are less than 1.5 mm, especially those with multilayer mimic structures, remains a challenge in vascular tissue engineering. In this study, poly(_L-lactide-co-caprolactone) (PLCL)/poly(Ɛ-caprolactone) (PCL)/tussah silk fibroin (TSF) SDVSs with a double-layer structure are prepared by one-step method based on friction twisting core-spun electrospinning technology. The constructed PLCL/PCL SDVSs grafted TSF have an obvious double-layer structure; tube wall thickness 524 ± 28 µm; and inner tube diameter 1390 ± 40 µm. Compared with traditional nanofiber vascular scaffolds (TS), the axial and radial tensile strengths of PLCL/PCL SDVSs grafted TSF increase by 86% and 34%, respectively. They also show good scaffold elastic recovery and burst pressure (BP) (8505 ± 875 mmHg). Compared with the PLCL/PCL SDVSs, the inner and outer layers of PLCL/PCL SDVSs grafted TSF show good hydrophilicity and protein adsorption performance. The in vitro cell viability results indicate that the inner and outer layers of PLCL/PCL SDVSs grafted TSF show enhanced proliferation and adhesion of vein endothelial cells (VECs) and smooth muscle cells (SMCs), respectively. Therefore, the successful preparation of PLCL/PCL SDVSs grafted TSF provides more possibilities for the clinical transplantation of small-diameter vascular scaffolds.

中文翻译：

用于血管组织工程的双层结构小直径 PLCL/PCL 纳米纤维接枝 TSF 血管支架

成功构建内径小于1.5 mm的小直径双层血管支架（SDVS），尤其是那些具有多层模拟结构的支架，仍然是血管组织工程中的一个挑战。在这项研究中，聚（_大号-lactide-合作己内酯）（PLCL）/聚（Ɛ-己内酯）（PCL）/柞蚕丝素蛋白（TSF）SDVSs采用基于摩擦捻芯纺静电纺丝技术的一步法制备具有双层结构。构建的PLCL/PCL SDVSs接枝TSF具有明显的双层结构；管壁厚度 524 ± 28 µm；和内管直径 1390 ± 40 µm。与传统的纳米纤维血管支架（TS）相比，PLCL/PCL SDVSs 接枝 TSF 的轴向和径向拉伸强度分别增加了 86% 和 34%。它们还显示出良好的支架弹性恢复和爆破压力 (BP) (8505 ± 875 mmHg)。与PLCL/PCL SDVSs相比，接枝TSF的PLCL/PCL SDVSs的内外层表现出良好的亲水性和蛋白质吸附性能。体外细胞活力结果表明，移植 TSF 的 PLCL/PCL SDVS 的内层和外层分别显示出静脉内皮细胞 (VEC) 和平滑肌细胞 (SMC) 的增殖和粘附增强。因此，PLCL/PCL SDVSs移植TSF的成功制备，为小直径血管支架的临床移植提供了更多的可能性。

更新日期：2021-11-15

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11