Salmonella is one of the most important pathogens involved in food intoxication outbreaks, and in many cases, the intoxication has been linked to shellfish which is typically consumed raw. While much is understood about the interactions between Salmonella and vertebrates, much less is known about its relationships with invertebrates, which could be an overlooked and important aspect to better understand the Salmonella interaction with its diversified hosts. The aim of this study was to investigate the effect of preadaptation in seawater microcosms during 12 months on Salmonella Typhimurium by determining its survival capacity within this mollusk over a period of 30 days. The results showed that the stressed bacteria are able to survive in this mollusk at a higher concentration even after thirty days of infection compared to bacteria in the normal state. In order to minimize the effect of an experimental device for one month on the survival of Salmonella, we carried out an in vitro study to determine the number of viable Salmonella in the hemocytes of oysters. Interestingly, we evaluated the effect of the antibacterial activity of different extracts of C. gigas using the solvents (Methanol, Ethanol and acetic acid) specifically against stressed and unstressed Salmonella. Furthermore, we compared the expression of three genes in the oyster Cg-big-def1, timp and sod in response to experimental infections of this mollusk with Vibrio splendidus kb133 and S. Typhimurium LT2DT104 in normal and stressed states. These findings are very important to contribute to explaining several questions about the persistence of S. Typhimurium for a long time in C. gigas and the host's immune response to this microorganism which is considered to be non-virulent for molluscs.

沙门氏菌是食物中毒爆发中最重要的病原体之一，在许多情况下，中毒与通常生食的贝类有关。虽然对沙门氏菌和脊椎动物之间的相互作用了解很多，但对其与无脊椎动物的关系知之甚少，这可能是一个被忽视的重要方面，可以更好地了解沙门氏菌与其多样化宿主的相互作用。本研究的目的是调查 12 个月内海水微观世界的预适应对沙门氏菌的影响通过确定其在 30 天内在该软体动物中的生存能力来确定鼠伤寒杆菌。结果表明，与正常状态下的细菌相比，即使在感染 30 天后，受压力的细菌也能够以更高的浓度在这种软体动物中存活。为了尽量减少实验装置一个月对沙门氏菌存活的影响，我们进行了一项体外研究，以确定牡蛎血细胞中活沙门氏菌的数量。有趣的是，我们评估了不同提取物的抗菌活性的效果C.牡蛎使用的溶剂（甲醇，乙醇和乙酸）特异性针对应激和未应激沙门氏菌. 此外，我们比较了在牡蛎三个基因的表达CG-大DEF1，TIMP和超氧化物歧化酶响应这个软体动物与实验感染灿烂弧菌kb133和S.鼠伤寒沙门氏菌LT2DT104在正常和受压的状态。这些发现非常重要，有助于解释关于持久性的几个问题S.鼠伤寒沙门氏菌在很长的时间长牡蛎和宿主对这种微生物被认为是无毒力的软体动物的免疫反应。