Glabridin is a natural plant-derived compound that has been widely used in medicine and cosmetic applications. However, the fungicidal mechanism of glabridin against phytopathogens remains unclear. In this study, we determined the biological activity and physiological effects of glabridin against F. graminearum. Then the differentially expressed proteins of F. graminearum were screened. The EC₅₀ values of glabridin in inhibiting the mycelial growth and conidial germination of F. graminearum were 110.70 mg/L and 40.47 mg/L respectively. Glabridin-induced cell membrane damage was indicated by morphological observations, DiBAC₄(3) and PI staining, and measurements of relative conductivity, ergosterol content and respiratory rates. These assays revealed that the integrity of the membrane was destroyed, the content of ergosterol decreased, and the respiratory rate was inhibited. A proteomics analysis showed that 186 proteins were up-regulated and 195 proteins were down-regulated. Mechanically sensitive ion channel proteins related to transmembrane transport and ergosterol biosynthesis ERG4/ERG24, related to ergosterol synthesis were blocked. It is speculated that glabridin acts on ergosterol synthesis-related proteins to destroy the integrity of the cell membrane, resulting in abnormal transmembrane transport and an increased membrane potential. Finally, the morphology of mycelia was seriously deformed, growth and development were inhibited. As a result death was even induced.

光甘草定是一种天然植物衍生化合物，已广泛用于医药和化妆品应用。然而，光甘草定对植物病原体的杀菌机制仍不清楚。在这项研究中，我们确定了光甘草定对禾谷镰刀菌的生物活性和生理作用。然后筛选出禾谷镰刀菌的差异表达蛋白。光甘草定抑制禾谷镰刀菌菌丝生长和分生孢子萌发的EC ₅₀值分别为110.70 mg/L和40.47 mg/L。形态学观察表明光甘草定诱导的细胞膜损伤，DiBAC ₄(3) 和 PI 染色，以及相对电导率、麦角甾醇含量和呼吸频率的测量。这些测定表明膜的完整性被破坏，麦角甾醇的含量降低，并且呼吸频率受到抑制。蛋白质组学分析表明，186 种蛋白质上调，195 种蛋白质下调。与跨膜转运和麦角甾醇生物合成ERG4/ERG24相关的机械敏感离子通道蛋白，有关麦角甾醇的合成被阻断。推测光甘草定作用于麦角甾醇合成相关蛋白，破坏细胞膜的完整性，导致跨膜转运异常，膜电位升高。最后，菌丝体形态严重变形，生长发育受到抑制。结果甚至导致死亡。