Classical gold standard HBV detection relies on expensive devices and complicated procedures, thus is always restricted in large-scale hospitals and centers for disease control and prevention. To extend HBV detection to primary clinics especially in underdeveloped areas, we design amplification-free smartphone-based attomolar HBV detecting technique based on single molecule sensing. Verified by synthesized HBV target DNA, this technique reaches a detection limit at attomolar concentration (100 aM); and verified by 110 clinical samples, it also reaches a rather high sensitivity of 10⁴ copy/mL (≈2000 IU/mL) with a high accuracy of 93.64% certificated by gold standard HBV detecting devices. Besides, this technique can quantify HBV viral load in 70 min only using portable and inexpensive devices as well as simple operations. Because of its cost-effective, field-portable and operable design, highly sensitive and selective detecting capability and wireless data connectivity, this technique can be potentially used in mobile HBV diagnoses and share HBV epidemic information especially in resource limited situations.

经典的金标准HBV检测依赖于昂贵的设备和复杂的程序，因此一直受限于大型医院和疾病预防控制中心。为了将 HBV 检测扩展到初级诊所，特别是在欠发达地区，我们设计了基于单分子传感的无扩增智能手机 attomolar HBV 检测技术。通过合成的HBV靶DNA验证，该技术在阿托摩尔浓度（100 aM）下达到检测限；并经110个临床样本验证，灵敏度达到10 ⁴copy/mL (≈2000 IU/mL) 具有 93.64% 的高精度，由金标准 HBV 检测设备证明。此外，该技术仅使用便携式和廉价的设备以及简单的操作就可以在 70 分钟内量化 HBV 病毒载量。由于其具有成本效益、现场便携和可操作的设计、高度灵敏和选择性的检测能力以及无线数据连接，该技术有可能用于移动 HBV 诊断和共享 HBV 流行信息，尤其是在资源有限的情况下。