The abuse of antibiotics has triggered the rise of drug-resistance bacteria, which has seriously threatened public health globally. As a result, carrying out efficient and accurate antibiotic and bacteria identification are quite significant but challenge. Herein, an unprecedented Cd-MOF-based sensor, [CdL]_n [1, H₂L = 4-(2-methyl-1H-benzo[d]imidazol-1-yl) isophthalic acid] with multiple fluorescence response behaviours towards antibiotics and bacteria was developed. Single-crystal X-ray diffraction revealed that 1 is a mesomeric 2D bilayer, which is comprised of two opposite chiral mono-layers, each assembled by left-handed or right-handed helixes. More interestingly, 1 represented multiplex detection capability towards antibiotics and bacteria through two detection behaviors: toward nitro-antibiotics and chlortetracycline (CTC) via fluorescent quenching, while toward Staphylococcus albus (S. albus) via fluorescent enhancement. Remarkably, 1 showed a low limit of detection (LOD, 47 CFU/mL) accompanied with specificity in the detection of S. albus compared to other bacteria, such as Staphylococcus aureus, Acinetobacter baumannii, Klebsiella pneumonia, Pseudomonas aeruginosa and Escherichia coli. In addition, the LOD could reach to ppm level for nitro-antibiotics and CTC. Moreover, the practical application of 1 was further reinforced through the detection of nitro-antibiotics and CTC, as well as S. albus in fetal calf serum and river water.

抗生素的滥用引发了耐药菌的增多，严重威胁了全球公共卫生。因此，进行高效、准确的抗生素和细菌鉴定非常重要，但也具有挑战性。在此，一种前所未有的基于 Cd-MOF 的传感器 [CdL] _n [ 1 , H ₂ L = 4-(2-methyl-1H-benzo[ d ]imidazol-1-yl) interphthalic acid] 具有多种荧光响应行为抗生素和细菌被开发出来。单晶 X 射线衍射显示1是介观二维双层，由两个相反的手性单层组成，每个单层由左旋或右旋螺旋组装而成。更有趣的是，1通过两种检测行为表示对抗生素和细菌的多重检测能力：通过荧光淬灭对硝基抗生素和金霉素（CTC），而通过荧光增强对白色葡萄球菌（S. albus）进行检测。值得注意的是，与其他细菌（如金黄色葡萄球菌、鲍曼不动杆菌、肺炎克雷伯菌、铜绿假单胞菌和大肠杆菌）相比， 1显示出低检测限（LOD，47 CFU/mL）和特异性检测白色念珠菌. 此外，硝基抗生素和 CTC 的 LOD 可达到 ppm 水平。此外，通过检测小牛血清和河水中的硝基抗生素和CTC，以及S. albus ，进一步加强了1的实际应用。