The discovery of male sterile materials is of great significance for the development of plant fertility research. Wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen) is a variety of non-heading Chinese cabbage. There are few studies on the male sterility of wucai, and the mechanism of male sterility is not clear. In this study, the male sterile mutant MS7–2 and the wild-type fertile plant MF7–2 were studied. Phenotypic characteristics and cytological analysis showed that MS7–2 abortion occurred at the tetrad period. The content of related sugars in the flower buds of MS7–2 was significantly lower than that of MF7–2, and a large amount of reactive oxygen species (ROS) was accumulated. Through transcriptome sequencing of MS7–2 and MF7–2 flower buds at three different developmental stages (a–c), 2865, 3847, and 4981 differentially expressed genes were identified in MS7–2 at the flower bud development stage, stage c, and stage e, respectively, compared with MF7–2. Many of these genes were enriched in carbohydrate metabolism, phenylpropanoid metabolism, and oxidative phosphorylation, and most of them were down-regulated in MS7–2. The down-regulation of genes involved in carbohydrate and secondary metabolite synthesis as well as the accumulation of ROS in MS7–2 led to pollen abortion in MS7–2. This study helps elucidate the mechanism of anther abortion in wucai, providing a basis for further research on the molecular regulatory mechanisms of male sterility and the screening and cloning of key genes in wucai.

中文翻译：

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五彩雄性不育突变体MS7-2不同花药发育阶段的形态特征和转录组分析

雄性不育材料的发现对植物育性研究的发展具有重要意义。五菜（Brassica campestris L. ssp. chinensis var. rosularis Tsen）是一种不结球白菜。关于五彩的雄性不育的研究较少，雄性不育的机制尚不明确。在本研究中，研究了雄性不育突变体 MS7-2 和野生型可育植物 MF7-2。表型特征和细胞学分析表明 MS7-2 流产发生在四分体时期。MS7-2花蕾中相关糖的含量显着低于MF7-2，并积累了大量的活性氧（ROS）。通过 MS7-2 和 MF7-2 花蕾在三个不同发育阶段 (a-c)、2865、3847、与MF7-2相比，MS7-2在花芽发育阶段、c阶段和e阶段分别鉴定了4981个差异表达基因。其中许多基因富含碳水化合物代谢、苯丙烷代谢和氧化磷酸化，其中大部分在 MS7-2 中下调。参与碳水化合物和次级代谢物合成的基因的下调以及 MS7-2 中 ROS 的积累导致 MS7-2 中的花粉流产。本研究有助于阐明五彩花药败育的机制，为进一步研究雄性不育的分子调控机制和五彩关键基因的筛选和克隆提供依据。其中许多基因富含碳水化合物代谢、苯丙烷代谢和氧化磷酸化，其中大部分在 MS7-2 中下调。参与碳水化合物和次级代谢物合成的基因的下调以及 MS7-2 中 ROS 的积累导致 MS7-2 中的花粉流产。本研究有助于阐明五彩花药败育的机制，为进一步研究雄性不育的分子调控机制和五彩关键基因的筛选和克隆提供依据。其中许多基因富含碳水化合物代谢、苯丙烷代谢和氧化磷酸化，其中大部分在 MS7-2 中下调。参与碳水化合物和次级代谢物合成的基因的下调以及 MS7-2 中 ROS 的积累导致 MS7-2 中的花粉流产。本研究有助于阐明五彩花药败育的机制，为进一步研究雄性不育的分子调控机制和五彩关键基因的筛选和克隆提供依据。参与碳水化合物和次级代谢物合成的基因的下调以及 MS7-2 中 ROS 的积累导致 MS7-2 中的花粉流产。本研究有助于阐明五彩花药败育的机制，为进一步研究雄性不育的分子调控机制和五彩关键基因的筛选和克隆提供依据。参与碳水化合物和次级代谢物合成的基因的下调以及 MS7-2 中 ROS 的积累导致 MS7-2 中的花粉流产。本研究有助于阐明五彩花药败育的机制，为进一步研究雄性不育的分子调控机制和五彩关键基因的筛选和克隆提供依据。