An original electrodeposited polymer film, based on 3-ethynylthiophene, for covalent immobilization of proteins via “click” reaction of copper catalyzed azide-alkyne cycloaddition was developed. The best characteristics have demonstrated the layer-by-layer immobilized 3,4-ethylendioxythiophene-3-ethynylthiophene composite film. The bovine serum albumin and antibodies have been successfully immobilized on the surface of planar platinum electrode. The approach has been applied to label-free electrochemical immunosensors for Escherichia coli and Staphylococcus aureus determination. The immunosensors demonstrated LODs 7.2 CFU/mL for Escherichia coli and 15.9 CFU/mL for Staphylococcus aureus, linear range 10²–10⁶ CFU/mL and could be promising for environmental and food control.

中文翻译：

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基于 3-乙炔噻吩的“可点击”电沉积聚合物薄膜，用于蛋白质的共价固定。无标记电化学免疫传感器在大肠杆菌和金黄色葡萄球菌检测中的应用

开发了一种基于 3-乙炔基噻吩的原始电沉积聚合物薄膜，用于通过铜催化的叠氮化物-炔环加成的“点击”反应共价固定蛋白质。最好的特性已经证明了逐层固定的 3,4-乙二氧基噻吩-3-乙炔基噻吩复合膜。牛血清白蛋白和抗体已成功固定在平面铂电极表面。该方法已应用于用于大肠杆菌和金黄色葡萄球菌测定的无标记电化学免疫传感器。免疫传感器显示大肠杆菌的LOD 为 7.2 CFU/ mL，金黄色葡萄球菌的LOD 为15.9 CFU/mL ，线性范围 10 ² –10⁶  CFU/mL，可用于环境和食品控制。