Background

Cardiovascular diseases (CVDs) are the most common and the first cause of death worldwide. While some studies have investigated the association of the Adenosine Deaminase (ADA) gene with CDVs, its roles on in-stent restenosis (ISR) has not been studied.

Methods and results

In this study, we investigated the role of ADA gene variants in both genetic and haplotype models on the risk of ISR. 91 samples were included in this study. The subjects were divided into two groups regarding having or not-having ISR (n = 40 ISR+ and n = 51 ISR−). The genotyping for G22A (rs73598374) and A4223C (rs452159) polymorphisms was performed using PCR–RFLP method. Statistical analysis was performed by SPSS v. 20 and Haploview 4.2 softwares. The basic demographic conditions in ISR groups were statistically similar. There was a significant association between A allele of rs452159 ISR groups after adjustment (allelic model: P value = 0.028, OR(95%CI) = 0.366(0.149–0.899)), while rs73598374 polymorphism shows no significant association with ISR. In haplotype analysis, the GA (G:rs73598374/A:rs452159) haplotype decreased the risk of ISR (P value = 00.025, OR(95%CI) = 0.382(0.161–0.907)).

Conclusions

This study suggests that A allele of ADA rs452159 polymorphism and GA (G:rs73598374/A:rs452159) haplotype may be related to decreased risk of ISR in CAD patients receiving drug-eluting stent and offers more observational studies on ADA variants in other populations to generate a potential haplotype panel for ISR risk assessment.

中文翻译：

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ADA基因单倍型与支架内冠状动脉再狭窄有关

背景

心血管疾病 (CVDs) 是全球最常见和首要的死亡原因。虽然一些研究调查了腺苷脱氨酶 ( ADA ) 基因与 CDV 的关系，但尚未研究其在支架内再狭窄 (ISR) 中的作用。

方法和结果

在这项研究中，我们研究了ADA基因变异在遗传和单倍型模型中对 ISR 风险的作用。本研究共纳入 91 个样本。受试者被分为两组，有或没有 ISR（n = 40 ISR+ 和 n = 51 ISR-）。使用 PCR-RFLP 方法对G22A ( rs73598374 ) 和A4223C ( rs452159 ) 多态性进行基因分型。通过SPSS v.20和Haploview 4.2软件进行统计分析。ISR 组的基本人口统计状况在统计学上相似。rs452159的A等位基因之间存在显着关联调整后的 ISR 组（等位基因模型：P 值 = 0.028，OR(95%CI) = 0.366(0.149–0.899)），而rs73598374多态性与 ISR 无显着关联。在单倍型分析中，GA ( G:rs73598374/A:rs452159 ) 单倍型降低了 ISR 的风险 (P 值 = 00.025, OR(95%CI) = 0.382(0.161–0.907))。

结论

本研究表明，ADA rs452159多态性和GA等位基因（G: rs73598374/ A :rs452159）单倍型可能与接受药物洗脱支架的 CAD 患者 ISR 风险降低有关，并为其他人群中的ADA变异提供更多观察性研究为 ISR 风险评估生成一个潜在的单倍型面板。