Tin derived antimony/nitrogen-doped porous carbon (Sb/NPC) composite for electrochemical sensing of albumin from hepatocellular carcinoma patients,Microchimica Acta

当前位置： X-MOL 学术 › Microchim. Acta › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Tin derived antimony/nitrogen-doped porous carbon (Sb/NPC) composite for electrochemical sensing of albumin from hepatocellular carcinoma patients
Microchimica Acta ( IF 5.3 ) Pub Date : 2021-09-12 , DOI: 10.1007/s00604-021-05005-7
Rabia Ashiq ₁ , Batool Fatima ₁ , Mohibullah Shah ₁ , Dilshad Hussain ₂ , Abrar Mohyuddin ₃ , Saadat Majeed ₄ , Rubaida Mehmood ₅ , Muhammad Imran ₆ , Muhammad Naeem Ashiq ₄ , Muhammad Najam-Ul-Haq ₄

Affiliation

An electrochemical sensor based on an antimony/nitrogen-doped porous carbon (Sb/NPC) composite has been developed for the quantitative detection of albumin from hepatocellular carcinoma (HCC) patients. Sb/NPC is hydrothermally synthesized from Sn/NPC precursors. The synthesized precursor (Sn/NPC) and the product (Sb/NPC) are characterized by XRD, FTIR, TGA, UV/Vis, SEM, and AFM. Cyclic voltammetry, chronoamperometry, and electrochemical impedance studies are used to investigate the electrochemical performance of Sb/NPC-GCE. Sb/NPC-GCE detects albumin at physiological pH of 7.4 in the potential range 0.92 V and 0.09 V for oxidation and reduction, respectively. LOD and recovery of Sb/NPC-GCE for the determination of albumin are 0.13 ng.mL⁻¹ and 66.6 ± 0.97–100 ± 2.73%, respectively. Chronoamperometry of the modified working electrode demonstrates its stability for 14 h, indicating its reusability and reproducibility. Sb/NPC-GCE is a selective sensor for albumin detection in the presence of interfering species. The electrode has been applied for albumin detection in human serum samples of HCC patients. A negative correlation of albumin with alpha-fetoprotein levels in HCC patients is observed by statistical analysis.

Graphical abstract

中文翻译：

锡衍生的锑/氮掺杂多孔碳 (Sb/NPC) 复合材料用于肝细胞癌患者白蛋白的电化学传感

基于锑/氮掺杂多孔碳 (Sb/NPC) 复合材料的电化学传感器已被开发用于定量检测肝细胞癌 (HCC) 患者的白蛋白。Sb/NPC 是由 Sn/NPC 前驱体水热合成的。通过XRD、FTIR、TGA、UV/Vis、SEM和AFM对合成的前驱体(Sn/NPC)和产物(Sb/NPC)进行了表征。循环伏安法、计时电流法和电化学阻抗研究用于研究 Sb/NPC-GCE 的电化学性能。Sb/NPC-GCE 在 0.92 V 和 0.09 V 的氧化和还原电位范围内分别检测生理 pH 值为 7.4 的白蛋白。用于测定白蛋白的 Sb/NPC-GCE 的 LOD 和回收率为 0.13 ng.mL ^-1和 66.6 ± 0.97–100 ± 分别为 2.73%。改性工作电极的计时电流法证明了其 14 小时的稳定性，表明其可重复使用性和再现性。Sb/NPC-GCE 是一种选择性传感器，用于在存在干扰物质的情况下检测白蛋白。该电极已应用于 HCC 患者的人血清样本中的白蛋白检测。通过统计分析观察到白蛋白与 HCC 患者的甲胎蛋白水平呈负相关。

图形概要

更新日期：2021-09-12

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11