This study aimed to find out the effective biotreatment processes of aqueous methyl parathion in stipulations of its biotransformation by potential indigenous mpd strains of Pseudomonas aeruginosa and Fusarium spp. Microbial biotransformation study of methyl parathion in contaminated synthetic wastewater at optimum conditions by P. aeruginosa mpd-5 (at temperature 33 °C, pH 7, under the aerobic condition with inoculum density of 10⁸cells/mL) and by Fusarium spp mpd-1 (at temperature 30 °C, pH 7, under the aerobic condition with inoculum density of 25 mgL⁻¹ dry biomass) was carried out. The major biodegradation compounds formed during the biotreatment process were analyzed by spectral studies using GCMS and FTIR. GCMS analysis of bacterial transformed compounds was p-nitrophenol, dimethylaminophenol, and glycyl-L-proline of 2-(3-methylpiperidino-4-5, 6-benzothiazin-4-one) and 2, 5-piperazinodione 3, 6-bis (2-methylpropyl). And the fungal transformed compounds were observed to be p-nitrophenol, phenol,2,4-Bis(1,1-Dimethylethyl), beta-L-arabinopyranoside-methyl, Cyclobutanethiol, 3-2(4)-thiophenone, dihydro-5-(1-methylethyl), Benzene,1,3-bis(1,1-dimethyl ethyl), Butanoic acid, 2-methyl methyl ester, and L-(+)-Ascorbic acid. The FTIR spectra of the molecule and the products of methyl parathion are observed to be changed in structures. It can be concluded from the aforementioned results and discussions that P. aeruginosa mpd-5 and Fusarium spp mpd-1 can be used in the biotreatment of pesticide wastewater having the high methyl parathion concentration and is possible by the indigenous microbial strains; they utilize as phosphate and carbon source of energy. Hence the strains can be used as a whole microbial cell, or its bioactive metabolites can be applied for the biotreatment of pesticide wastewater and potentially degrade methylparathion.

本研究旨在通过潜在的铜绿假单胞菌和镰刀 菌属的本地 mpd 菌株的生物转化来确定甲基对硫磷水溶液的有效生物处理过程。在最佳条件下，铜绿假单胞菌mpd-5（温度 33 °C，pH 7，有氧条件下，接种密度为 10 ^{8 个}细胞/mL）和镰刀菌属mpd-对受污染合成废水中甲基对硫磷的微生物生物转化研究1（温度30°C，pH 7，有氧条件下，接种密度为25 mgL ^-1干生物量）。在生物处理过程中形成的主要生物降解化合物通过光谱研究使用 GCMS 和 FTIR 进行分析。细菌转化化合物的 GCMS 分析是对硝基苯酚、二甲氨基苯酚和 2-(3-methylpiperidino-4-5, 6-benzothiazin-4-one) 和 2, 5-哌嗪二酮 3, 6-bis 的甘氨酰-L-脯氨酸(2-甲基丙基)。并且观察到真菌转化的化合物是对硝基苯酚、苯酚、2,4-双(1,1-二甲基乙基)、β-L-阿拉伯吡喃糖苷-甲基、环丁硫醇、3-2(4)-噻吩酮、二氢-5 -(1-甲基乙基)、苯、1,3-双(1,1-二甲基乙基)、丁酸、2-甲基甲酯和L-(+)-抗坏血酸。观察到分子和甲基对硫磷产物的 FTIR 光谱在结构上发生了变化。P。aeruginosa mpd-5和Fusarium spp mpd-1可用于高甲基对硫磷浓度的农药废水的生物处理，可以通过本地微生物菌株进行生物处理；它们用作磷酸盐和碳能源。因此，这些菌株可以作为一个完整的微生物细胞使用，或者其生物活性代谢物可以用于农药废水的生物处理，并可能降解甲基对硫磷。