• Plants are able to detect insect eggs deposited on leaves. In Arabidopsis, eggs of the butterfly species Pieris brassicae (common name large white) induce plant defenses and activate the salicylic acid (SA) pathway. We previously discovered that oviposition triggers a systemic acquired resistance (SAR) against the bacterial hemibiotroph pathogen Pseudomonas syringae.
• Here, we show that insect eggs or treatment with egg extract (EE) induce SAR against the fungal necrotroph Botrytis cinerea BMM and the oomycete pathogen Hyaloperonospora arabidopsidis Noco2. This response is abolished in ics1, ald1 and fmo1, indicating that the SA pathway and the N-hydroxypipecolic acid (NHP) pathway are involved.
• Establishment of EE-induced SAR in distal leaves potentially involves tryptophan-derived metabolites, including camalexin. Indeed, SAR is abolished in the biosynthesis mutants cyp79B2 cyp79B3, cyp71a12 cyp71a13 and pad3-1, and camalexin is toxic to B. cinerea in vitro.
• This study reveals an interesting mechanism by which lepidopteran eggs interfere with plant–pathogen interactions.

中文翻译：

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昆虫卵引发对真菌和卵菌病原体的系统获得性抗性

• 植物能够检测沉积在叶子上的昆虫卵。在拟南芥中，蝴蝶物种Pieris brassicae（俗称大白）的卵诱导植物防御并激活水杨酸 (SA) 通路。我们之前发现，产卵会引发针对细菌半生物营养病原体丁香假单胞菌的系统获得性抗性 (SAR) 。
• 在这里，我们表明昆虫卵或用卵提取物 (EE) 处理可诱导 SAR 针对真菌坏死菌Botrytis cinerea BMM 和卵菌病原体Hyaloperonospora arabidopsidis Noco2。这种反应在ics1、ald1和fmo1中被废除，表明涉及 SA 途径和 N-羟基哌啶酸 (NHP) 途径。
• 在远端叶片中建立 EE 诱导的 SAR 可能涉及色氨酸衍生代谢物，包括 camalexin。事实上，SAR 在生物合成突变体cyp79B2 cyp79B3、cyp71a12 cyp71a13和pad3-1中被废除，并且 camalexin在体外对 B. cinerea 有毒。
• 这项研究揭示了鳞翅目昆虫卵干扰植物与病原体相互作用的有趣机制。