Clausena lansium (Lour.) Skeels (Rutaceae), recognized as wampee, is a widely distributed fruit tree which is utilized as a folk-medicine for treatment of several common diseases. However, the genomic information about this medicinally important species is still lacking. Therefore, we assembled the first genome of Clausena genus with a total length of 310.51 Mb and scaffold N50 of 2.24 Mb by using 10× Genomics technology. Further annotation revealed a total of 34,419 protein-coding genes, while repetitive elements covered 39.08% (121.36 Mb) of the genome. The Clausena and Citrus genus were found to diverge around 22 MYA, and also shared an ancient whole-genome triplication event with Vitis. Furthermore, multi-tissue transcriptomic analysis enabled the identification of genes involved in the synthesis of carbazole alkaloids. Altogether, these findings provided new insights into the genome evolution of Wampee species and highlighted the possible role of key genes involved in the carbazole alkaloids biosynthetic pathway.

Clausena lansium (Lour.) Skeels (芸香科)，被认为是黄皮，是一种分布广泛的果树，被用作治疗几种常见疾病的民间药物。然而，仍然缺乏关于这种具有重要药用价值的物种的基因组信息。因此，我们利用10×Genomics技术组装了Clausena属的第一个基因组，总长度为310.51 Mb，支架N50为2.24 Mb。进一步的注释揭示了总共 34,419 个蛋白质编码基因，而重复元素覆盖了基因组的 39.08% (121.36 Mb)。发现Clausena和Citrus属在22 MYA 左右出现分歧，并且还与Vitis共享了一个古老的全基因组三倍体事件。此外，多组织转录组分析能够鉴定参与咔唑生物碱合成的基因。总之，这些发现为黄皮物种的基因组进化提供了新的见解，并强调了咔唑生物碱生物合成途径中涉及的关键基因的可能作用。