The assessment of glutathione (GSH) levels is associated with early diagnostics and pathological analysis for various disorders. Among all kinds of techniques for detecting GSH, the colorimetric assay relying on the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) catalyzed by many nanomaterials with peroxidase-like activity attracts increasing attention owing to its outstanding merits, such as high sensitivity and high selectivity. However, the aggregation between the nanomaterials severely hinders the entrance of TMB into the “active site” of these peroxidase mimics. To address this problem, the D-amino acid incorporated nanoflowers possessing peroxidase-like activity with a diameter of 10–15 μm, TMB and H₂O₂ were employed to establish the detection system for determining the level of glutathione. The larger diameter size of the hybrid nanoflowers substantially averts the aggregation between them. The results confirm that the hybrid nanoflowers detection system presents a low limit of detection, wide linear range, perfect selectivity, good storage stability and desired operational stability for the detection of GSH relying on the intrinsic peroxidase-like activity and favorable mechanical stability of the hybrid nanoflowers, indicating that the hybrid nanoflowers detection system has tremendous application potential in clinical diagnosis and treatment.

中文翻译：

---

具有内在过氧化物酶样活性的氨基酸掺入纳米花的制备及其在以TMB自由基阳离子为指示剂有效测定谷胱甘肽中的应用

谷胱甘肽 (GSH) 水平的评估与各种疾病的早期诊断和病理分析有关。在各种检测 GSH 的技术中，依赖于 3,3',5,5'-四甲基联苯胺 (TMB) 氧化的比色法在许多具有过氧化物酶活性的纳米材料的催化下受到越来越多的关注，例如如高灵敏度和高选择性。然而，纳米材料之间的聚集严重阻碍了 TMB 进入这些过氧化物酶模拟物的“活性位点”。为了解决这个问题，D-氨基酸掺入了具有过氧化物酶活性的直径为 10-15 μm、TMB 和 H ₂ O _{2 的}纳米花建立了谷胱甘肽水平检测系统。混合纳米花的较大直径尺寸基本上避免了它们之间的聚集。结果证实，杂化纳米花检测系统具有检测下限低、线性范围宽、选择性好、储存稳定性好、操作稳定性好等特点，依赖于杂合体固有的类过氧化物酶活性和良好的机械稳定性检测谷胱甘肽。纳米花，表明混合纳米花检测系统在临床诊断和治疗中具有巨大的应用潜力。