Banana Fusarium wilt, caused by the fungus pathogen Fusarium oxysporum f. sp. cubense (Foc), is a devastating disease that causes tremendous reductions in banana yield worldwide. Secreted proteins can act as pathogenicity factors and play important roles in the Foc–banana interactions. In this study, a shotgun-based proteomic approach was employed to characterize and compare the secretomes of Foc1 and Foc4 upon banana extract treatment, which detected 1183 Foc1 and 2450 Foc4 proteins. Comprehensive in silico analyses further identified 447 Foc1 and 433 Foc4 proteins in the classical and non-classical secretion pathways, while the remaining proteins might be secreted through currently unknown mechanisms. Further analyses showed that the secretomes of Foc1 and Foc4 are similar in their overall functional characteristics and share largely conserved repertoires of CAZymes and effectors. However, we also identified a number of potentially important pathogenicity factors that are differentially present in Foc1 and Foc4, which may contribute to their different pathogenicity against banana hosts. Furthermore, our quantitative PCR analysis revealed that genes encoding secreted pathogenicity factors differ significantly between Foc1 and Foc4 in their expression regulation in response to banana extract treatment. To our knowledge, this is the first experimental secretome analysis that focused on the pathogenicity mechanism in different Foc races. The results of this study provide useful resources for further exploration of the complicated pathogenicity mechanisms in Foc.

中文翻译：

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体外分泌组分析表明尖孢镰刀菌 f 之间存在差异的致病机制。sp。立方第 1 场和第 4 场比赛

香蕉枯萎病，由真菌病原体Fusarium oxysporum f 引起。sp。立方(Foc)，是一种毁灭性的疾病，导致全球香蕉产量大幅下降。分泌的蛋白质可以作为致病因子并在 Foc-香蕉相互作用中发挥重要作用。在这项研究中，采用基于鸟枪法的蛋白质组学方法来表征和比较香蕉提取物处理后 Foc1 和 Foc4 的分泌组，检测到 1183 个 Foc1 和 2450 个 Foc4 蛋白质。全面的计算机分析进一步确定了经典和非经典分泌途径中的 447 个 Foc1 和 433 个 Foc4 蛋白，而其余蛋白质可能通过目前未知的机制分泌。进一步的分析表明，Foc1 和 Foc4 的分泌组在它们的整体功能特征上是相似的，并且在很大程度上共享 CAZymes 和效应子的保守库。然而，我们还确定了一些潜在的重要致病因子，它们在 Foc1 和 Foc4 中存在差异，这可能导致它们对香蕉宿主的致病性不同。此外，我们的定量 PCR 分析显示，编码分泌性致病因子的基因在 Foc1 和 Foc4 之间响应香蕉提取物处理的表达调控存在显着差异。据我们所知，这是第一个专注于不同 Foc 种族致病机制的实验性分泌组分析。本研究结果为进一步探索 Foc 中复杂的致病机制提供了有用的资源。我们的定量 PCR 分析显示，编码分泌性致病因子的基因在 Foc1 和 Foc4 之间响应香蕉提取物处理的表达调控存在显着差异。据我们所知，这是第一个专注于不同 Foc 种族致病机制的实验性分泌组分析。本研究结果为进一步探索 Foc 中复杂的致病机制提供了有用的资源。我们的定量 PCR 分析显示，编码分泌性致病因子的基因在 Foc1 和 Foc4 之间响应香蕉提取物处理的表达调控存在显着差异。据我们所知，这是第一个专注于不同 Foc 种族致病机制的实验性分泌组分析。本研究结果为进一步探索 Foc 中复杂的致病机制提供了有用的资源。