The calmodulin-binding transcription activator (CAMTA), as one of the most distinctive families of transcription factors, plays an important role in plant growth and development and in the stress response. However, it is currently unknown whether CAMTA exists in cucumbers and what its function is. In this study, we first identified four CAMTA genes in the cucumber genome using a genome-wide search method. Subsequently, we analyzed their physical and chemical properties, gene structure, protein domains, and phylogenetic relationships. The results show that the structure of CsCAMTAs is similar to that of other plants, and a phylogenetic analysis divides them into three groups. The analysis of cis-acting elements shows that most CsCAMTAs contain a variety of hormones and stress-related elements. The RT-PCR analysis shows that CsCAMTAs have different expression levels in different tissues and can be induced by IAA, ABA, MeJA, NaCl, and PEG. Finally, we analyzed the expression pattern of CsCAMTAs’ alternative spliceosomes under salt and drought stress. The results show that the expression levels of the different spliceosomes are affected by the type of stress and the duration of stress. These data indicate that CsCAMTAs participate in growth and development and in the stress response in cucumbers, a finding which lays the foundation for future CsCAMTAs’ functional research.

中文翻译：

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黄瓜 (Cucumis sativus L.) CAMTA 家族基因的全基因组鉴定、表达谱和选择性剪接分析

钙调蛋白结合转录激活因子（CAMTA）作为最独特的转录因子家族之一，在植物生长发育和逆境响应中发挥着重要作用。然而，目前尚不清楚黄瓜中是否存在CAMTA及其功能。在这项研究中，我们首先使用全基因组搜索方法在黄瓜基因组中鉴定了四个CAMTA基因。随后，我们分析了它们的理化性质、基因结构、蛋白质结构域和系统发育关系。结果表明，CsCAMTAs的结构与其他植物相似，系统发育分析将它们分为三类。顺式作用元件的分析表明，大多数CsCAMTA包含多种激素和压力相关元素。RT-PCR分析表明，CsCAMTAs在不同组织中具有不同的表达水平，可被IAA、ABA、MeJA、NaCl和PEG诱导。最后，我们分析了盐和干旱胁迫下CsCAMTAs 的选择性剪接体的表达模式。结果表明，不同剪接体的表达水平受应激类型和应激持续时间的影响。这些数据表明CsCAMTAs参与黄瓜的生长发育和应激反应，这一发现为未来CsCAMTAs 的功能研究奠定了基础。