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Epilactose Biosynthesis Using Recombinant Cellobiose 2-Epimerase Produced by Saccharomyces cerevisiae
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-09-10 , DOI: 10.1021/acsfoodscitech.1c00183 Beatriz B. Cardoso 1 , Sara C. Silvério 1 , Joana L. Rodrigues 1 , Lígia R. Rodrigues 1
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-09-10 , DOI: 10.1021/acsfoodscitech.1c00183 Beatriz B. Cardoso 1 , Sara C. Silvério 1 , Joana L. Rodrigues 1 , Lígia R. Rodrigues 1
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Cellobiose 2-epimerases are interesting enzymes that can catalyze the production of lactose-based prebiotics, namely, epilactose and lactulose, through a single substrate reaction with noteworthy yields. In this work, the GRAS yeast Saccharomyces cerevisiae was for the first time used as a host for the heterologous production of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus. The enzyme showed an optimal temperature and an optimal pH of 80 °C and 7.5, respectively. Under these conditions, the time-course production of epilactose from lactose (50 g/L) was evaluated. The maximum yield and productivity obtained were 27% and 9 g L–1 h–1, respectively. This epilactose yield represents the highest value obtained from the several approaches reported using cellobiose 2-epimerase from C. saccharolyticus. In conclusion, it was shown that the enzyme produced by S. cerevisiae is suitable for the production of epilactose-enriched prebiotic mixtures with potential applications in the food industry.
中文翻译：
使用酿酒酵母生产的重组纤维二糖 2-差向异构酶生物合成表乳糖
纤维二糖 2-差向异构酶是一种有趣的酶，可以通过单一底物反应催化生产基于乳糖的益生元，即表乳糖和乳果糖，产率显着。在这项工作中，GRAS 酵母Saccharomyces cerevisiae首次用作异源生产来自Caldicellulosiruptor saccharolyticus的纤维二糖 2-差向异构酶的宿主。该酶的最适温度和最适 pH 值分别为 80 °C 和 7.5。在这些条件下，评估了从乳糖 (50 g/L) 生产外乳糖的时间过程。获得的最大产量和生产率为 27% 和 9 g L –1 h –1， 分别。这种表乳糖产量代表了从使用来自C. saccharolyticus 的纤维二糖 2-差向异构酶报道的几种方法中获得的最高值。总之，表明由酿酒酵母产生的酶适用于生产富含表乳糖的益生元混合物，在食品工业中具有潜在应用。
更新日期：2021-10-15
中文翻译：
使用酿酒酵母生产的重组纤维二糖 2-差向异构酶生物合成表乳糖
纤维二糖 2-差向异构酶是一种有趣的酶，可以通过单一底物反应催化生产基于乳糖的益生元，即表乳糖和乳果糖，产率显着。在这项工作中，GRAS 酵母Saccharomyces cerevisiae首次用作异源生产来自Caldicellulosiruptor saccharolyticus的纤维二糖 2-差向异构酶的宿主。该酶的最适温度和最适 pH 值分别为 80 °C 和 7.5。在这些条件下，评估了从乳糖 (50 g/L) 生产外乳糖的时间过程。获得的最大产量和生产率为 27% 和 9 g L –1 h –1， 分别。这种表乳糖产量代表了从使用来自C. saccharolyticus 的纤维二糖 2-差向异构酶报道的几种方法中获得的最高值。总之，表明由酿酒酵母产生的酶适用于生产富含表乳糖的益生元混合物，在食品工业中具有潜在应用。
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