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Inorganic Additive Improves the Survival of the Probiotic Lacticaseibacillus rhamnosus CRL1505 During Spray Drying, Rehydration, and Storage
Current Microbiology ( IF 2.3 ) Pub Date : 2021-09-11 , DOI: 10.1007/s00284-021-02648-w
M A Correa Deza 1 , L Díaz Vergara 2 , S Salva 1 , M A Montenegro 2 , G Font de Valdez 1 , C L Gerez 1
Affiliation  

In previous in vitro studies, an inorganic additive (MCM3) showed a thermo-protective effect on the cell viability of Lacticaseibacillus rhamnosus CRL1505 (Lr-CRL1505). In this work, cultures of this probiotic strain were spray dried at lab scale using two carriers: maltodextrin (powder MA) and maltodextrin plus MCM3 (powder MA/MCM3). The cell survival was higher in powder MA/MCM3 (72.8%) than in powder MA (42.8%). Different rehydration media, including the additive MCM3, and two temperatures (37 °C and 45 °C) were evaluated. The best results were obtained in cells rehydrated at 37 °C in MCM3. During the storage of the powders, the highest cell counts were observed in the MA/MCM3 powder. Our results demonstrated that the presence of MCM3 in the carrier and in reconstitution media benefits the spray drying process and the recovery of dehydrated cells. Thus, the use of this additive of inorganic nature and low cost represents a promising technological alternative.



中文翻译:

无机添加剂可提高益生菌鼠李糖乳杆菌 CRL1505 在喷雾干燥、再水化和储存过程中的存活率

在之前的体外研究中,无机添加剂 (MCM 3 ) 显示出对鼠李糖杆菌CRL1505 (Lr-CRL1505)细胞活力的热保护作用。在这项工作中,这种益生菌菌株的培养物在实验室规模下使用两种载体进行喷雾干燥:麦芽糊精(粉末 MA)和麦芽糊精加 MCM 3(粉末 MA/MCM 3)。粉末 MA/MCM 3 (72.8%) 中的细胞存活率高于粉末 MA (42.8%)。评估了不同的再水化介质,包括添加剂 MCM 3和两个温度(37 °C 和 45 °C)。在 MCM 3 中37 °C 再水化的细胞中获得了最佳结果. 在粉末储存期间,在 MA/MCM 3粉末中观察到最高的细胞计数。我们的结果表明,MCM 3在载体和重构培养基中的存在有利于喷雾干燥过程和脱水细胞的回收。因此,使用这种无机性质和低成本的添加剂代表了一种有前途的技术替代方案。

更新日期:2021-09-12
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