In previous in vitro studies, an inorganic additive (MCM₃) showed a thermo-protective effect on the cell viability of Lacticaseibacillus rhamnosus CRL1505 (Lr-CRL1505). In this work, cultures of this probiotic strain were spray dried at lab scale using two carriers: maltodextrin (powder MA) and maltodextrin plus MCM₃ (powder MA/MCM₃). The cell survival was higher in powder MA/MCM₃ (72.8%) than in powder MA (42.8%). Different rehydration media, including the additive MCM₃, and two temperatures (37 °C and 45 °C) were evaluated. The best results were obtained in cells rehydrated at 37 °C in MCM₃. During the storage of the powders, the highest cell counts were observed in the MA/MCM₃ powder. Our results demonstrated that the presence of MCM₃ in the carrier and in reconstitution media benefits the spray drying process and the recovery of dehydrated cells. Thus, the use of this additive of inorganic nature and low cost represents a promising technological alternative.

在之前的体外研究中，无机添加剂 (MCM ₃ ) 显示出对鼠李糖乳杆菌CRL1505 (Lr-CRL1505)细胞活力的热保护作用。在这项工作中，这种益生菌菌株的培养物在实验室规模下使用两种载体进行喷雾干燥：麦芽糊精（粉末 MA）和麦芽糊精加 MCM ₃（粉末 MA/MCM ₃）。粉末 MA/MCM ₃ (72.8%) 中的细胞存活率高于粉末 MA (42.8%)。评估了不同的再水化介质，包括添加剂 MCM ₃和两个温度（37 °C 和 45 °C）。在 MCM _{3 中}37 °C 再水化的细胞中获得了最佳结果. 在粉末储存期间，在 MA/MCM ₃粉末中观察到最高的细胞计数。我们的结果表明，MCM ₃在载体和重构培养基中的存在有利于喷雾干燥过程和脱水细胞的回收。因此，使用这种无机性质和低成本的添加剂代表了一种有前途的技术替代方案。