Aphids and their diverse symbionts have become a good model to study bacteria-arthropod symbiosis. The feeding habits of aphids are usually influenced by a variety of symbionts. Most studies on symbiont diversity have focused on polyphagous aphids, while symbiont community patterns for oligophagous aphids remain unclear. Here, we surveyed the bacterial communities in natural populations of two oligophagous aphids, Melanaphis sacchari and Neophyllaphis podocarpi, in natural populations. Seven common symbionts were detected, among which Buchnera aphidicola and Wolbachia were the most prevalent. In addition, an uncommon Sodalis-like symbiont was also detected in these two aphids, and Gilliamella was found in some samples of M. sacchari. We further assessed the significant variation in symbiont communities within the two aphid species, geographical regions and host specialization using statistical and ordination analyses. Geography was an important factor in shaping the symbiont community structure in these oligophagous aphids. Furthermore, the strong geographical influence may be related to specific environmental factors, especially temperature, among different regions. These findings extend our knowledge of the significance of geography and its associated environmental conditions in the symbiont community structure associated with oligophagous aphids.

中文翻译：

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两种寡食蚜虫物种的地理依赖共生群落

蚜虫及其多样的共生体已成为研究细菌-节肢动物共生的良好模型。蚜虫的食性通常受多种共生体的影响。大多数关于共生体多样性的研究都集中在多食性蚜虫上，而寡食性蚜虫的共生体群落模式仍不清楚。在这里，我们调查了自然种群中两种寡食性蚜虫Melanaphis sacchari和Neophyllaphis podocarppi 的自然种群中的细菌群落。检测到7种常见的共生体，其中以Buchnera aphidicola和Wolbachia最为普遍。此外，在这两种蚜虫中还检测到了一种不常见的类Sodalis共生体，Gilliamella在M. sacchari 的一些样品中发现。我们使用统计和排序分析进一步评估了两种蚜虫物种、地理区域和宿主专业化内共生群落的显着变化。地理是塑造这些寡食蚜虫共生群落结构的重要因素。此外，强烈的地理影响可能与不同地区之间特定的环境因素有关，尤其是温度。这些发现扩展了我们对地理及其相关环境条件在与寡食蚜虫相关的共生群落结构中的重要性的认识。