ABSTRACT

Increasing evidence shows that immune-related genes (IRGs) play an important role in bariatric surgery (BS). We identified differentially expressed immune-related genes (DEIRGs) of adipose tissue after BS by analysing the two expression profiles of GEO (GSE59034 and GSE29409). Subsequently, enrichment analysis, GSEA and PPI networks were examined to identify the hub IRGs and related pathways. The performance of the signature was evaluated by area under the curve (AUC) of the receiver operating characteristic (ROC). CIBERSORT algorithm was used to evaluate the relative abundance of infiltrated immune cells.42 DEIRGs were found between the GSE59034 and GSE29409 datasets. The AUC of the signature was 0.904 and 0.865 in the GSE58979 and GSE48452, respectively. Interestingly, the signature also showed good performance in diagnosing non-alcoholic fatty liver disease (NAFLD) (AUC was 0.834 and 0.800, respectively). The number of neutrophils, macrophages M2, macrophages M0 and dendritic cells activated decreased significantly. After BS, the infiltration of T cells regulatory, monocytes, mast cells resting and plasma cells in adipose tissue increased. The novel proposed IRGs signature reveals the underlying immune mechanism of BS and is a promising biomarker for distinguishing the severity of NAFLD. This will provide new insights into strategies for treating obesity and NAFLD.

摘要

该签名在诊断非酒精性脂肪肝（NAFLD）方面也表现出良好的性能（AUC 分别为 0.834 和 0.800）。中性粒细胞、巨噬细胞M2、巨噬细胞M0和活化的树突细胞数量明显减少。BS后，脂肪组织中调节性T细胞、单核细胞、肥大细胞和浆细胞的浸润增加。新提出的 IRGs 特征揭示了 BS 的潜在免疫机制，是区分 NAFLD 严重程度的有前途的生物标志物。这将为治疗肥胖和 NAFLD 的策略提供新的见解。脂肪组织中静止的肥大细胞和浆细胞增加。新提出的 IRGs 特征揭示了 BS 的潜在免疫机制，是区分 NAFLD 严重程度的有前途的生物标志物。这将为治疗肥胖和 NAFLD 的策略提供新的见解。脂肪组织中静止的肥大细胞和浆细胞增加。新提出的 IRGs 特征揭示了 BS 的潜在免疫机制，是区分 NAFLD 严重程度的有前途的生物标志物。这将为治疗肥胖和 NAFLD 的策略提供新的见解。