A platform based on cryogel monoliths in small capillaries, which allows very strong enrichment of an analyte through a capture and release process, is described. For their preparation, a photoreactive copolymer solution containing capture molecules of interest is filled into a capillary, frozen in, and then photochemically transformed into cryogel monoliths through C,H-insertion cross-linking reactions. As a test example, the platform is used for the preconcentration of dopamine from bovine serum albumin and urine samples through capture and release processes. During capture from a large volume and release into a smaller volume, the platform shows recovery rates up to 97% and allows up to a roughly 630-fold enrichment of the concentration of the analyte. The presented platform could be used as a disposable device for the purification and enrichment of a variety of cis-diol-containing samples.

中文翻译：

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用于通过捕获和释放富集分析物的冷冻凝胶整体

描述了一种基于小毛细管中的冷冻凝胶整体的平台，该平台允许通过捕获和释放过程非常强烈地富集分析物。在制备过程中，将含有感兴趣捕获分子的光反应共聚物溶液填充到毛细管中，冷冻，然后通过 C,H 插入交联反应光化学转化为冷冻凝胶整体。作为测试示例，该平台用于通过捕获和释放过程从牛血清白蛋白和尿液样本中预浓缩多巴胺。在从大体积捕获并释放到小体积期间，该平台显示出高达 97% 的回收率，并使分析物的浓度增加大约 630 倍。