Thiols are important units in amino acids such as cysteine and peptides like glutathione. Development of chemical sensors capable of precise detection of thiols is important in cancer diagnosis and therapy. We have developed novel two-photon fluorescent turn-on probes for selective detection of thiols. The probes displayed excellent sensitivity and low detection limits. The dual-purpose probes have been demonstrated to be suitable for simultaneous imaging and proteome profiling in live cells and tumor tissues. The unique turn-on design endows the probes with excellent selectivity toward thiols in vitro and in situ, and can be further developed to support a thiol-quantification assay.

中文翻译：

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用于高效检测和分析活细胞和组织中的硫醇的双光子荧光开启探针

硫醇是半胱氨酸等氨基酸和谷胱甘肽等肽中的重要单位。开发能够精确检测硫醇的化学传感器在癌症诊断和治疗中非常重要。我们开发了用于选择性检测硫醇的新型双光子荧光开启探针。探针显示出优异的灵敏度和低检测限。两用探针已被证明适用于活细胞和肿瘤组织中的同时成像和蛋白质组分析。独特的开启设计使探针对硫醇具有出色的选择性体外和原位，并且可以进一步开发以支持硫醇定量测定。