Quercetin (QCT) is an effective antioxidant, antifibrotic and wound healing agent. Silver nanoparticles (AgNPs) are an effective antimicrobial, antifungal and wound healing agent and considered as gold standard for wound treatment especially diabetic and burn wounds. The present study aimed to investigate QCT loaded AgNPs in hydrogel matrices (QCT-AgNPs hydrogel) as synergistic treatment paradigms for diabetic wound. Quality by Design approach was employed for the optimization of hydrogel preparation using carbopol-934 and aloevera. The developed QCT-AgNPs hydrogel was characterized for hydrodynamic diameter, %entrapment efficiency (%EE), surface morphology, texture analysis, in-vitro drug release, skin irritation study, ex-vivo permeation study (confocal study), and antimicrobial efficacy. The optimized formulation showed hydrodynamic diameter of ∼44.1 nm with smooth spherical surface morphology and ∼92.09% of QCT was entrapped in QCT-AgNPs hydrogel matrices. The antimicrobial study revealed superior therapeutic efficacy of QCT-AgNPs hydrogel in comparison to marketed (MRKT) gel on S. aureus and E. coli. Moreover, in-vivo results demonstrated that QCT-AgNPs hydrogel significantly (p<0.001) reduced the wound gap and increased % re-epithelialization compared with diabetic control after 18 d of post treatment in excisional diabetic wound model. In conclusion, this study opens up an avenue for the treatment of diabetic wound.

槲皮素 (QCT) 是一种有效的抗氧化剂、抗纤维化和伤口愈合剂。银纳米粒子 (AgNPs) 是一种有效的抗菌剂、抗真菌剂和伤口愈合剂，被认为是伤口治疗的金标准，尤其是糖尿病和烧伤伤口。本研究旨在研究水凝胶基质（QCT-AgNPs 水凝胶）中负载 QCT 的 AgNPs 作为糖尿病伤口的协同治疗范例。质量源于设计的方法被用于优化使用 carbopol-934 和芦荟的水凝胶制备。开发的 QCT-AgNPs 水凝胶对流体动力学直径、包封率 (%EE)、表面形态、质地分析、体外药物释放、皮肤刺激研究、离体进行了表征渗透研究（共聚焦研究）和抗菌功效。优化后的配方显示出约 44.1 nm 的流体动力学直径，具有光滑的球形表面形态，约 92.09% 的 QCT 被困在 QCT-AgNPs 水凝胶基质中。抗菌研究表明，与市售的 (MRKT) 凝胶相比，QCT-AgNPs 水凝胶对金黄色葡萄球菌和大肠杆菌的治疗效果更好。此外，体内结果表明，在切除糖尿病伤口模型中，与糖尿病对照相比，QCT-AgNPs 水凝胶显着（ p <0.001）减少了伤口间隙并增加了再上皮化百分比。总之，本研究为糖尿病伤口的治疗开辟了一条途径。