Lipases, particularly microbial lipases, are important industrial biocatalysts. As a result, lipase enzyme screening, synthesis, and purification from microbial strains are constantly evolving to meet the needs of the pharmaceutical and food industries. Thus, the goal of this study was to identify the most potential lipase-producing bacterial strains from Aavin dairy industry effluent contaminated soil. Furthermore, growth parameters, such as pH, temperature, carbon and nitrogen sources, were optimized for lipase enzyme production from selected bacterial strains. According to the findings, 9 strains (V1–V9) of 15 bacterial isolates were found to be lipase producers. However, three strains (V1, V7, and V8) predominated and demonstrated significant lipase-producing activity. These V1, V7, and V8 bacterial strains were identified as Bacillus pumilus V1, Bacillus pumilus V7, and Bacillus subtilis V8 through 16S rRNA sequencing. About 16.6 to 27.8 µg mL⁻¹ of lipase production was recorded under the optimal growth conditions: pH 8, temperature 37 °C, fructose and yeast extract as suitable carbon and nitrogen source. Among these 3 strains B. pumilus V1 showed excellent lipase productivity than others. The molecular weight of this lipase produced by bacteria was determined to be 35 kDa using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE).

脂肪酶，特别是微生物脂肪酶，是重要的工业生物催化剂。因此，从微生物菌株中筛选、合成和纯化脂肪酶的方法不断发展，以满足制药和食品行业的需求。因此，本研究的目标是从 Aavin 乳制品工业废水污染的土壤中鉴定出最具潜力的产脂肪酶菌株。此外，生长参数，如 pH 值、温度、碳源和氮源，针对从选定的细菌菌株生产脂肪酶进行了优化。根据研究结果，发现 15 个细菌分离株中的 9 个菌株 (V1-V9) 是脂肪酶生产者。然而，三个菌株（V1、V7 和 V8）占主导地位并表现出显着的脂肪酶生产活性。这些 V1、V7 和 V8 菌株被鉴定为短小芽孢杆菌V1、短小芽孢杆菌V7 和枯草芽孢杆菌V8 通过 16S rRNA 测序。在最佳生长条件下记录了约 16.6 至 27.8 µg mL ^-1的脂肪酶产量：pH 8、温度 37 °C、果糖和酵母提取物作为合适的碳源和氮源。在这 3 个菌株中，短小芽孢杆菌V1 显示出比其他菌株优异的脂肪酶生产力。使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 确定细菌产生的这种脂肪酶的分子量为 35 kDa。